Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv

Kyung Hee Rhee; Ki Seog Lee; Amit Priyadarshi; Eunice Eunkyung Kim; Kwang Yeon Hwang

doi:10.1107/S1744309105034330

Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv

Kyung Hee Rhee, Ki Seog Lee, Amit Priyadarshi, Eunice Eunkyung Kim, Kwang Yeon Hwang

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P3₁ or P3₂, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 Å³ Da^-1. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.

Original language	English
Pages (from-to)	1017-1019
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	61
Issue number	11
DOIs	https://doi.org/10.1107/S1744309105034330
Publication status	Published - 2005

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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10.1107/S1744309105034330

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Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv. / Rhee, Kyung Hee; Lee, Ki Seog; Priyadarshi, Amit; Kim, Eunice Eunkyung; Hwang, Kwang Yeon.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 11, 2005, p. 1017-1019.

Research output: Contribution to journal › Article › peer-review

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title = "Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv",

abstract = "Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P31 or P32, with unit-cell parameters a = b = 109.56, c = 147.97 {\AA}. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 {\AA}3 Da-1. A complete native data set has been collected at 2.7 {\AA} resolution using a synchrotron-radiation source.",

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AU - Rhee, Kyung Hee

AU - Lee, Ki Seog

AU - Priyadarshi, Amit

AU - Kim, Eunice Eunkyung

AU - Hwang, Kwang Yeon

PY - 2005

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AB - Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P31 or P32, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 Å3 Da-1. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.

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Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv

Abstract

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UN SDGs

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