Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv

Kyung H. Rhee; Ki Seog Lee; Amit Priyadarshi; Eunice Eunkyung Kim; Kwang Yeon Hwang

doi:10.1107/S1744309105034330

Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv

Kyung H. Rhee, Ki Seog Lee, Amit Priyadarshi, Eunice Eunkyung Kim, Kwang Yeon Hwang

Division of Biotechnology

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P3₁ or P3₂, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 Å³ Da^-1. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.

Original language	English
Pages (from-to)	1017-1019
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	61
Issue number	11
DOIs	https://doi.org/10.1107/S1744309105034330
Publication status	Published - 2005 Dec 1

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ASJC Scopus subject areas

Biochemistry
Biophysics
Genetics
Structural Biology
Condensed Matter Physics

Cite this

Rhee, K. H., Lee, K. S., Priyadarshi, A., Kim, E. E., & Hwang, K. Y. (2005). Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 61(11), 1017-1019. https://doi.org/10.1107/S1744309105034330

Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv. / Rhee, Kyung H.; Lee, Ki Seog; Priyadarshi, Amit; Kim, Eunice Eunkyung; Hwang, Kwang Yeon.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 11, 01.12.2005, p. 1017-1019.

Research output: Contribution to journal › Article

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10.1107/S1744309105034330