Abstract
Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P31 or P32, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 Å3 Da-1. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.
| Original language | English |
|---|---|
| Pages (from-to) | 1017-1019 |
| Number of pages | 3 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Volume | 61 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2005 |
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Genetics
- Condensed Matter Physics
Fingerprint Dive into the research topics of 'Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv'. Together they form a unique fingerprint.
Cite this
Rhee, K. H., Lee, K. S., Priyadarshi, A., Kim, E. E., & Hwang, K. Y. (2005). Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 61(11), 1017-1019. https://doi.org/10.1107/S1744309105034330