TY - JOUR
T1 - Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv
AU - Rhee, Kyung Hee
AU - Lee, Ki Seog
AU - Priyadarshi, Amit
AU - Kim, Eunice Eunkyung
AU - Hwang, Kwang Yeon
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005
Y1 - 2005
N2 - Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P31 or P32, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 Å3 Da-1. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.
AB - Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P31 or P32, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 Å3 Da-1. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.
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U2 - 10.1107/S1744309105034330
DO - 10.1107/S1744309105034330
M3 - Article
C2 - 16511223
AN - SCOPUS:33744496308
VL - 61
SP - 1017
EP - 1019
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
SN - 1744-3091
IS - 11
ER -