Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv

Kyung H. Rhee, Ki Seog Lee, Amit Priyadarshi, Eunice Eunkyung Kim, Kwang Yeon Hwang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P31 or P32, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 Å3 Da-1. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.

Original languageEnglish
Pages (from-to)1017-1019
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number11
DOIs
Publication statusPublished - 2005 Dec 1

Fingerprint

Racemases and Epimerases
tuberculosis
fatty acids
Coenzyme A
Crystallization
Mycobacterium tuberculosis
Fatty Acids
X-Rays
crystallization
X rays
x rays
Stereoisomerism
Synchrotron radiation
Synchrotrons
radiation sources
Monomers
Vapors
homogeneity
glycols
polyethylenes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology
  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv. / Rhee, Kyung H.; Lee, Ki Seog; Priyadarshi, Amit; Kim, Eunice Eunkyung; Hwang, Kwang Yeon.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 11, 01.12.2005, p. 1017-1019.

Research output: Contribution to journalArticle

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