Abstract
Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P31 or P32, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 Å3 Da-1. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.
| Original language | English |
|---|---|
| Pages (from-to) | 1017-1019 |
| Number of pages | 3 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Volume | 61 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2005 Dec 1 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Genetics
- Structural Biology
- Condensed Matter Physics
Cite this
Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv. / Rhee, Kyung H.; Lee, Ki Seog; Priyadarshi, Amit; Kim, Eunice Eunkyung; Hwang, Kwang Yeon.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 11, 01.12.2005, p. 1017-1019.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv
AU - Rhee, Kyung H.
AU - Lee, Ki Seog
AU - Priyadarshi, Amit
AU - Kim, Eunice Eunkyung
AU - Hwang, Kwang Yeon
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P31 or P32, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 Å3 Da-1. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.
AB - Fatty acid-CoA racemase plays an important role in the β-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P31 or P32, with unit-cell parameters a = b = 109.56, c = 147.97 Å. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 Å3 Da-1. A complete native data set has been collected at 2.7 Å resolution using a synchrotron-radiation source.
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UR - http://www.scopus.com/inward/citedby.url?scp=33744496308&partnerID=8YFLogxK
U2 - 10.1107/S1744309105034330
DO - 10.1107/S1744309105034330
M3 - Article
C2 - 16511223
AN - SCOPUS:33744496308
VL - 61
SP - 1017
EP - 1019
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
SN - 1744-3091
IS - 11
ER -