Crystallization and preliminary X‐ray crystallographic analysis of arylesterase from Pseudomonas fluorescens

Kyeong Kyu Kim, Kwang Yeon Hwang, Kang Duk Choi, Joo Hyun Kang, Ook Joon Yoo, Se Won Suh

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Large crystals of arylesterase from Pseudomonas fluorescens have been grown at room temperature using ammonium sulfate as a precipitant. They grow to dimensions of 0.7 × 0.7 × 0.6 mm3 within a month. The crystals belong to the trigonal space group P31 (or P32), with unit cell dimensions of a= 147.12 Å and c= 131.08 Å. The asymmetric unit seems to contain six molecules of dimeric aryles‐terase, with corresponding crystal volume per protein mass (VM) of 2.53 Å3/Da and solvent fraction of 51.5% by volume. The crystals diffract to at least 2.2 Å Bragg spacing when exposed to X‐rays from a rotating‐anode source. X‐ray data have been collected to 2.9 Å Bragg spacing from native crystals. © 1993 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)213-215
Number of pages3
JournalProteins: Structure, Function, and Bioinformatics
Volume15
Issue number2
DOIs
Publication statusPublished - 1993 Feb
Externally publishedYes

Keywords

  • X‐ray diffraction
  • bacterial esterase
  • crystals

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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