CTG triplet repeats from human hereditary diseases are dominant genetic expansion products in Escherichia coli

Keiichi Ohshima, Seong Man Kang, Robert D. Wells

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The relative ability of the 10 triplet repeat sequences to be expanded in Escherichia coli was determined. Surprisingly, CTG tracts are expanded at least 8 times more frequently than any of the other nine triplets. Low levels of expansion were found also for CGG, GTG, and GTC. Thus, the structure of the CTG repeats and/or their utilization by the DNA synthetic systems in vivo must be quite different from the other triplets. These data further validate this genetically defined system for elucidating molecular mechanisms of expansion and may explain why most triplet repeat hereditary neuromuscular and neurodegenerative disease genes contain CTG repeats.

Original languageEnglish
Pages (from-to)1853-1856
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number4
DOIs
Publication statusPublished - 1996 Jan 26
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

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