CTL recognition of Qa-1b binding a peptide from preproinsulin leader sequences and characterization of Qa-1 from the NOD mouse

Carla J. Aldrich, Taehoon Chun, H. Rex Gaskins, Evan Hermel

Research output: Contribution to journalArticle

Abstract

Cytotoxic T lymphocyte (CTL) recognition of major histocompatibility complex (MHC) class I molecules binding peptides is important to the proper functioning of the immune system. Evidence indicates that the MHC class IB molecule Qa-1b, (encoded by H2-T23b) presents antigens to αβ and γδ T cells and that lymphocytes restricted to Qa-1 may play a role in immune regulation. We demonstrate that Qa-1b binds a nonamer peptide derived from the leader sequence of preproinsulin II. Alloreactive anti-Qa-1b CTL lyse Qa-1 plus insulin peptide bearing cells. Blocking assays further demonstrate that this peptide binds in the peptide binding cleft of Qa-1b. DNA sequencing of Qa-1 from the NOD (non obese diabetic, H-2g7) mouse shows that this strain expresses Qa-1a. However, alloreactive CTL recognition of Qa-1 on NOD cells may be unusually influenced by the available peptide pool. In addition, NIT-1, a pancreatic β cell line derived from the NOD mouse is not recognized by anti-Qa-1a CTL, whereas anti-Qa-1b CTL lyse βTC6-F7, a pancreatic line from a normal mouse strain. βTC6 and NIT-1 cells both were killed by anti-H2 class IA CTL. Thus, Qa-1 expression and peptide presentation, particularly on pancreatic β cells may be important in the regulation of autoimmune responses.

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
Publication statusPublished - 1998 Mar 20
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this