Curcumin protected PC12 cells against beta-amyloid-induced toxicity through the inhibition of oxidative damage and tau hyperphosphorylation

So Young Park, Hyo Shin Kim, Eun Kyung Cho, Bo Youn Kwon, Sohee Phark, Kwang W. Hwang, Dong Geun Sul

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

One of the pathological hallmarks of Alzheimer's disease is the progressive accumulation of beta-amyloid (Aβ) in the form of senile plaques, and Aβ insult to neuronal cells has been identified as one of the major causes of the onset of the disease. Curcumin, the major and most active antioxidant of Curcuma longa, protects neuronal cells against Aβ-induced toxicity. Therefore, in this study, we investigated the neuroprotective mechanisms by which curcumin acts against Aβ (25-35)-induced toxicity in PC12 cells. Following the exposure of PC12 cells to 10 μM Aβ (25-35) for 24 h, significant increases in the level of antioxidant enzymes, and DNA damage were observed, and these increases were accompanied by a decrease in cell viability, and an increase in intracellular calcium levels and tau hyperphosphorylation. In addition, pretreatment of PC12 cells with 10 μg/ml curcumin for 1 h significantly reversed the effect of Aβ, by decreasing the oxidative stress, and DNA damage induced by Aβ, as well as attenuating the elevation of intracellular calcium levels and tau hyperphosphorylation induced by Aβ. Taken together, these data indicate that curucmin protected PC12 cells against Aβ-induced neurotoxicity through the inhibition of oxidative damage, intracellular calcium influx, and tau hyperphosphorylation.

Original languageEnglish
Pages (from-to)2881-2887
Number of pages7
JournalFood and Chemical Toxicology
Volume46
Issue number8
DOIs
Publication statusPublished - 2008 Aug 1

Fingerprint

Curcumin
curcumin
PC12 Cells
amyloid
Amyloid
Toxicity
toxicity
Calcium
calcium
DNA damage
Antioxidants
neurons
DNA Damage
antioxidants
Curcuma longa
Oxidative stress
neurotoxicity
DNA
cells
Alzheimer disease

Keywords

  • Beta-amyloid
  • Calcium influx
  • Curcumin
  • Oxidative stress
  • Tau hyperphosphorylation

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Curcumin protected PC12 cells against beta-amyloid-induced toxicity through the inhibition of oxidative damage and tau hyperphosphorylation. / Park, So Young; Kim, Hyo Shin; Cho, Eun Kyung; Kwon, Bo Youn; Phark, Sohee; Hwang, Kwang W.; Sul, Dong Geun.

In: Food and Chemical Toxicology, Vol. 46, No. 8, 01.08.2008, p. 2881-2887.

Research output: Contribution to journalArticle

Park, So Young ; Kim, Hyo Shin ; Cho, Eun Kyung ; Kwon, Bo Youn ; Phark, Sohee ; Hwang, Kwang W. ; Sul, Dong Geun. / Curcumin protected PC12 cells against beta-amyloid-induced toxicity through the inhibition of oxidative damage and tau hyperphosphorylation. In: Food and Chemical Toxicology. 2008 ; Vol. 46, No. 8. pp. 2881-2887.
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