Curcumin Shows Antiviral Properties against Norovirus

Minji Yang, Gil Jae Lee, Jiyeon Si, Sung Joon Lee, Hyun Ju You, Gwang Pyo Ko

Research output: Contribution to journalArticle

Abstract

Phytochemicals provide environmentally friendly and relatively inexpensive natural products, which could potentially benefit public health by controlling human norovirus (HuNoV) infection. In this study, 18 different phytochemicals were evaluated for antiviral effects against norovirus using murine norovirus (MNV) as a model for norovirus biology. Among these phytochemicals, curcumin (CCM) was the most potent anti-noroviral phytochemical, followed by resveratrol (RVT). In a cell culture infection model, exposure to CCM or RVT for 3 days reduced infectivity of norovirus by 91% and 80%, respectively. To confirm the antiviral capability of CCM, we further evaluated its antiviral efficacy at various doses (0.25, 0.5, 0.75, 1, and 2 mg/mL) and durations (short-term: 10, 30, 60, and 120 min; long-term: 1, 3, 7, and 14 days). The anti-noroviral effect of CCM was verified to occur in a dose-dependent manner. Additionally, we evaluated the inhibitory effect of each phytochemical on the replication of HuNoV using a HuNoV replicon-bearing cell line (HG23). Neither CCM nor RVT had a strong inhibitory effect on HuNoV replication, which suggests that their antiviral mechanism may involve viral entry or other life cycle stages rather than the replication of viral RNA. Our results demonstrated that CCM may be a promising candidate for development as an anti-noroviral agent to prevent outbreaks of foodborne illness.

Original languageEnglish
JournalMolecules (Basel, Switzerland)
Volume21
Issue number10
Publication statusPublished - 2016 Oct 20

Fingerprint

Norovirus
Curcumin
Phytochemicals
Antiviral Agents
infectious diseases
public health
dosage
Bearings (structural)
biology
cultured cells
entry
Replicon
Viral RNA
Public health
Biological Products
Cell culture
cycles
Foodborne Diseases
Life cycle
products

Keywords

  • curcumin
  • norovirus
  • phytochemical

ASJC Scopus subject areas

  • Medicine(all)
  • Organic Chemistry

Cite this

Yang, M., Lee, G. J., Si, J., Lee, S. J., You, H. J., & Ko, G. P. (2016). Curcumin Shows Antiviral Properties against Norovirus. Molecules (Basel, Switzerland), 21(10).

Curcumin Shows Antiviral Properties against Norovirus. / Yang, Minji; Lee, Gil Jae; Si, Jiyeon; Lee, Sung Joon; You, Hyun Ju; Ko, Gwang Pyo.

In: Molecules (Basel, Switzerland), Vol. 21, No. 10, 20.10.2016.

Research output: Contribution to journalArticle

Yang, M, Lee, GJ, Si, J, Lee, SJ, You, HJ & Ko, GP 2016, 'Curcumin Shows Antiviral Properties against Norovirus', Molecules (Basel, Switzerland), vol. 21, no. 10.
Yang, Minji ; Lee, Gil Jae ; Si, Jiyeon ; Lee, Sung Joon ; You, Hyun Ju ; Ko, Gwang Pyo. / Curcumin Shows Antiviral Properties against Norovirus. In: Molecules (Basel, Switzerland). 2016 ; Vol. 21, No. 10.
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