Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells

Moon Sook Woo, Soo Hyun Jung, So Young Kim, Jin W. Hyun, Kwang H. Ko, Won-Ki Kim, Hee S. Kim

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

The aberrant expression of matrix metalloproteinase-9 (MMP-9) is implicated in the invasion and angiogenesis process of brain tumor. This study has investigated the effects of curcumin on MMP-9 expression in human astroglioma cell lines. Curcumin significantly inhibited the MMP-9 enzymatic activity and protein expression that was induced by PMA. The inhibitory effect of curcumin on MMP-9 expression correlates with the decreased MMP-9 mRNA level and the suppression of MMP-9 promoter activity. The curcumin-mediated inhibition of MMP-9 gene expression appears to occur via NF-κB and AP-1 because their DNA binding activities were suppressed by curcumin. Furthermore, curcumin strongly repressed the PMA-induced phosphorylation of ERK, JNK, and p38 MAP kinase, which were dependent on the PKC pathway. Therefore, the inhibition of MMP-9 expression by curcumin might have therapeutic potential for controlling the growth and invasiveness of brain tumor.

Original languageEnglish
Pages (from-to)1017-1025
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume335
Issue number4
DOIs
Publication statusPublished - 2005 Oct 7
Externally publishedYes

Fingerprint

Curcumin
Matrix Metalloproteinase 9
Astrocytoma
Phorbol Esters
Brain Neoplasms
Tumors
Brain
Phosphorylation
Extracellular Signal-Regulated MAP Kinases
Transcription Factor AP-1
p38 Mitogen-Activated Protein Kinases
Gene expression
Cells
Gene Expression
Cell Line
Messenger RNA
DNA
Growth

Keywords

  • AP-1
  • Astroglioma
  • Curcumin
  • Gene expression
  • MAP kinase
  • Matrix metalloproteinase-9
  • NF-κB
  • PKC
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Curcumin suppresses phorbol ester-induced matrix metalloproteinase-9 expression by inhibiting the PKC to MAPK signaling pathways in human astroglioma cells. / Woo, Moon Sook; Jung, Soo Hyun; Kim, So Young; Hyun, Jin W.; Ko, Kwang H.; Kim, Won-Ki; Kim, Hee S.

In: Biochemical and Biophysical Research Communications, Vol. 335, No. 4, 07.10.2005, p. 1017-1025.

Research output: Contribution to journalArticle

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