Curcumin suppresses proliferation and migration and induces apoptosis on human placental choriocarcinoma cells via ERK1/2 and SAPK/JNK MAPK signaling pathways

Whasun Lim, Muhah Jeong, Fuller W. Bazer, Gwonhwa Song

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Curcumin, a natural pigment for yellow color that originates from turmeric, is a diarylheptanoid widely studied for its antiinflammatory, antiangiogenic, antioxidant, and anticancer effects on cells. In placental diseases, including preeclampsia and preterm birth, curcumin reduces proinflammatory cytokines. Even though curcumin is regarded as a novel chemotherapeutic agent with strong apoptotic effects based on phenolic structure, little is known about its functional effects on choriocarcinoma. Therefore, in the present study, we investigated the chemotherapeutic effects of curcumin on choriocarcinoma cells (JAR and JEG3), which are valuable placental models. The results showed that curcumin decreased viability of choriocarcinoma cells in a dose-dependent manner. In addition, proliferative and migratory characteristics of JAR and JEG3 cells were inhibited by curcumin treatment and curcumin-induced apoptotic effects, which were assessed using TUNEL and annexin V/propidium iodide staining. Moreover, curcumin increased depolarization of the mitochondrial membrane based on JC-1 staining and changed expression of apoptotic proteins. Phosphorylation of mitogen-activated protein kinases (MAPK) responsible for regulation of anticancer effects of curcumin were examined for dose- and timedependent effects. The ERK1/2 and SAPK/JNK and their downstream molecules including P90RSK and c-Jun, respectively, were activated by curcumin. Moreover, pharmacological inhibitors of ERK1/2 (U0126) and SAPK/JNK (SP600125) suppressed ERK1/2 and SAPK/JNK activation respectively, and blockage of P38 MAPK by its inhibitor (SB203580) had a synergistic effect with curcumin. These results indicate that curcumin acts as a novel chemotherapeutic agent on human placental choriocarcinoma cells via activation of ERK1/2 and SAPK/JNK signal transduction cascades.

Original languageEnglish
Article number83
JournalBiology of Reproduction
Volume95
Issue number4
DOIs
Publication statusPublished - 2016 Oct 1

Fingerprint

Choriocarcinoma
Curcumin
JNK Mitogen-Activated Protein Kinases
Apoptosis
Diarylheptanoids
Placenta Diseases
Staining and Labeling
Curcuma
Propidium
Annexin A5
Premature Birth
In Situ Nick-End Labeling
Mitochondrial Membranes
p38 Mitogen-Activated Protein Kinases
Protein Kinase Inhibitors
Pre-Eclampsia
Mitogen-Activated Protein Kinases
Signal Transduction
Cell Survival
Anti-Inflammatory Agents

Keywords

  • Apoptosis
  • Chemoprevention
  • Choriocarcinoma
  • Curcumin
  • MAPK

ASJC Scopus subject areas

  • Cell Biology

Cite this

Curcumin suppresses proliferation and migration and induces apoptosis on human placental choriocarcinoma cells via ERK1/2 and SAPK/JNK MAPK signaling pathways. / Lim, Whasun; Jeong, Muhah; Bazer, Fuller W.; Song, Gwonhwa.

In: Biology of Reproduction, Vol. 95, No. 4, 83, 01.10.2016.

Research output: Contribution to journalArticle

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