Current and future techniques for detecting oxytocin: Focusing on genetically-encoded GPCR sensors

Dongmin Lee, Hyung Bae Kwon

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Oxytocin is a neuropituitary hormone that is involved in a wide range of psychosocial behaviors. Despite its psychophysiological importance as a neuromodulator in the CNS, effective techniques capable of monitoring oxytocin dynamics or testing related behavioral consequences are limited. Along with an explosive advancement in synthetic biology, high-performance genetically-encoded neuromodulator sensors are being developed. Here we comprehensively review the current methodologies available for detecting oxytocin in neuroscience. Their strengths and weaknesses are discussed, and a graphical summary is plotted for better comparison of techniques. We also suggest future directions for next generation oxytocin sensor development and their working principles.

Original languageEnglish
Article number109407
JournalJournal of Neuroscience Methods
Volume366
DOIs
Publication statusPublished - 2022 Jan 15

Keywords

  • Arrestin
  • Circularly permuted fluorescent protein
  • Enzyme complementation
  • Fast-scan cyclic voltammetry
  • G protein-coupled receptor
  • Gene expression
  • Genetically encoded sensor
  • Microdialysis
  • Neuromodulator
  • Neuropeptide
  • Oxytocin
  • Split luciferase
  • TEV protease

ASJC Scopus subject areas

  • Neuroscience(all)

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