CXCL14 enhances proliferation and migration of NCI-H460 human lung cancer cells overexpressing the glycoproteins containing heparan sulfate or sialic acid

Cho Rong Park, Dong Joo You, Dong Kyu Kim, Mi Jin Moon, Cheolju Lee, Seung Hyun Oh, Curie Ahn, Jae Young Seong, Jong-Ik Hwang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

CXCL14 is a chemokine family member that is involved in various cellular responses in addition to immune cell activation. Although constitutive CXCL14 expression in normal epithelial cells may help protect against infection by activating immune systems, its expression in cancer cells has raised controversy regarding its possible role in tumorigenesis. However, the underlying mechanisms for this disparity remain unknown. Investigation of cellular CXCL14 binding properties might increase our understanding of the peptide's roles in tumorigenesis. In the present study, we found that CXCL14 binds to various cell types. Interestingly, binding to NCI-H460 cells was prevented by heparan sulfate and N-acetyl neuraminic acid. Next, we examined effect of CXCL14 binding in NCI-H460 and NCI-H23. CXCL14 enhanced proliferation and migration in NCI-H460 but had no effect on NCI-H23. A reporter gene assay with various transcription factor response elements revealed that only nuclear factor-κB (NF-κB) signaling was activated by CXCL14 in NCI-H460 cells, which was blocked by BAPTA-AM, TPCA-1, and brefeldin A. Exogenous expression of some glycoproteins such as syndecan-4, podoplanin, and CD43 in these cells enhanced CXCL14 binding and NF-κB activity. Collectively, these results demonstrate that CXCL14 binding to glycoproteins harboring heparan sulfate proteoglycans and sialic acids leads proliferation and migration of some cancer cells.

Original languageEnglish
Pages (from-to)1084-1096
Number of pages13
JournalJournal of Cellular Biochemistry
Volume114
Issue number5
DOIs
Publication statusPublished - 2013 May 1

Fingerprint

Heparitin Sulfate
N-Acetylneuraminic Acid
Lung Neoplasms
Glycoproteins
Syndecan-4
Neuraminic Acids
Cells
Sialic Acids
Brefeldin A
Heparan Sulfate Proteoglycans
Immune system
Response Elements
Chemokines
Assays
Transcription Factors
Genes
Chemical activation
Peptides
Carcinogenesis
Reporter Genes

Keywords

  • CXCL14
  • glycoprotein
  • heparan sulfate proteoglycan
  • migration
  • NF-κB
  • sialic acid

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

CXCL14 enhances proliferation and migration of NCI-H460 human lung cancer cells overexpressing the glycoproteins containing heparan sulfate or sialic acid. / Park, Cho Rong; You, Dong Joo; Kim, Dong Kyu; Moon, Mi Jin; Lee, Cheolju; Oh, Seung Hyun; Ahn, Curie; Seong, Jae Young; Hwang, Jong-Ik.

In: Journal of Cellular Biochemistry, Vol. 114, No. 5, 01.05.2013, p. 1084-1096.

Research output: Contribution to journalArticle

Park, Cho Rong ; You, Dong Joo ; Kim, Dong Kyu ; Moon, Mi Jin ; Lee, Cheolju ; Oh, Seung Hyun ; Ahn, Curie ; Seong, Jae Young ; Hwang, Jong-Ik. / CXCL14 enhances proliferation and migration of NCI-H460 human lung cancer cells overexpressing the glycoproteins containing heparan sulfate or sialic acid. In: Journal of Cellular Biochemistry. 2013 ; Vol. 114, No. 5. pp. 1084-1096.
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