Cytochrome P450

Taming a wild type enzyme

Sang Taek Jung, Ryan Lauchli, Frances H. Arnold

Research output: Contribution to journalReview article

164 Citations (Scopus)

Abstract

Protein engineering of cytochrome P450 monooxygenases (P450s) has been very successful in generating valuable non-natural activities and properties, allowing these powerful catalysts to be used for the synthesis of drug metabolites and in biosynthetic pathways for the production of precursors of artemisinin and paclitaxel. Collected experience indicates that the P450s are highly 'evolvable' - they are particularly robust to mutation in their active sites and readily accept new substrates and exhibit new selectivities. Their ability to adapt to new challenges upon mutation may reflect the nonpolar nature of their active sites as well as their high degree of conformational variability.

Original languageEnglish
Pages (from-to)809-817
Number of pages9
JournalCurrent Opinion in Biotechnology
Volume22
Issue number6
DOIs
Publication statusPublished - 2011 Dec 1
Externally publishedYes

Fingerprint

Metabolites
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Catalytic Domain
Enzymes
Proteins
Protein Engineering
Mutation
Catalysts
Biosynthetic Pathways
Substrates
Paclitaxel
Pharmaceutical Preparations
artemisinine

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Cytochrome P450 : Taming a wild type enzyme. / Jung, Sang Taek; Lauchli, Ryan; Arnold, Frances H.

In: Current Opinion in Biotechnology, Vol. 22, No. 6, 01.12.2011, p. 809-817.

Research output: Contribution to journalReview article

Jung, Sang Taek ; Lauchli, Ryan ; Arnold, Frances H. / Cytochrome P450 : Taming a wild type enzyme. In: Current Opinion in Biotechnology. 2011 ; Vol. 22, No. 6. pp. 809-817.
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