Cytokine expression in placenta-derived mesenchymal stem cells in patients with pre-eclampsia and normal pregnancies

Jong Ha Hwang, Mi Jung Lee, Oye Sun Seok, Yu Chin Paek, Geum Joon Cho, Hyun Joo Seol, Jae Kwan Lee, Min Jeong Oh

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Objectives: The aim of this study was to investigate the levels of cytokines in placenta-derived mesenchymal stem cells (MSCs) in normal pregnancies and those with pre-eclampsia. Materials and methods: C5a, CD40 Ligand, G-CSF, GM-CSF, GROα, I-309, sICAM-1, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-16, IL-17, IL-17E, IL-23, IL-27, IL-32α, IP-10, I-TAC, MCP-1, MIF, MIP-1α, MIP-1β, Serpin E1, RANTES, SDF-1, TNFα, and sTREM-1 were measured in mesenchymal stem cells using the human cytokine array panel A. The soluble intracellular adhesion molecule-1 (sICAM-1), stromal-derived factor-1 (SDF-1) and monocyte chemotactic protein-1 (MCP-1) were measured by real-time PCR and confirmed by Western blot analysis. Results: MSCs derived from the deciduas of normal pregnancies had significantly elevated levels of sICAM (p = 0.000) and SDF-1 (p = 0.011), compared to the pregnancies with pre-eclampsia. The level of MCP-1 in the decidua-derived MSCs was not significantly different. No significant difference was observed between normal and pre-eclamptic pregnancies for the amnion-derived MSCs. Conclusions: The decreased levels of sICAM and SDF-1 found in the decidua-derived MSCs from pre-eclamptic pregnancies might be associated with some of the immunological alterations in pre-eclampsia.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalCytokine
Volume49
Issue number1
DOIs
Publication statusPublished - 2010 Jan

Keywords

  • Amnion
  • Cytokines
  • Decidua
  • Mesenchymal stem cells
  • Placenta

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Cytokine expression in placenta-derived mesenchymal stem cells in patients with pre-eclampsia and normal pregnancies'. Together they form a unique fingerprint.

  • Cite this

    Hwang, J. H., Lee, M. J., Seok, O. S., Paek, Y. C., Cho, G. J., Seol, H. J., Lee, J. K., & Oh, M. J. (2010). Cytokine expression in placenta-derived mesenchymal stem cells in patients with pre-eclampsia and normal pregnancies. Cytokine, 49(1), 95-101. https://doi.org/10.1016/j.cyto.2009.08.013