Cytoplasmic Protein Imaging with Mid-Infrared Photothermal Microscopy

Cellular Dynamics of Live Neurons and Oligodendrocytes

Jong Min Lim, Chanjong Park, Jin Sung Park, Changho Kim, Bonghwan Chon, Minhaeng Cho

Research output: Contribution to journalArticle

Abstract

Mid-infrared photothermal microscopy has been suggested as an alternative to conventional infrared microscopy because in addition to the inherent chemical contrast available upon vibrational excitation, it can feasibly achieve spatial resolution at the submicrometer level. Furthermore, it has substantial potential for real-time bioimaging for the observation of cellular dynamics without photodamage or photobleaching of fluorescent labels. We performed real-time imaging of oligodendrocytes to investigate cellular dynamics throughout the life cycle of a cell, revealing details of cell division and apoptosis, as well as cellular migration. In the case of live neurons, we observed a photothermal contrast associated with traveling protein complexes on an axon, which correspond to the transport of vesicles from the cell body to the dendritic branches of the neuron through the cytoskeleton. We anticipate that mid-infrared photothermal imaging will be of great use for gaining insights into the field of biophysical science, especially with regard to cellular dynamics and functions.

Original languageEnglish
JournalJournal of Physical Chemistry Letters
DOIs
Publication statusPublished - 2019 Jan 1

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Oligodendroglia
neurons
Neurons
Microscopy
Microscopic examination
microscopy
Infrared radiation
proteins
Photobleaching
Proteins
Imaging techniques
Transport Vesicles
Life Cycle Stages
Cytoskeleton
Cells
Cell Division
cell division
Axons
axons
Observation

ASJC Scopus subject areas

  • Materials Science(all)
  • Physical and Theoretical Chemistry

Cite this

Cytoplasmic Protein Imaging with Mid-Infrared Photothermal Microscopy : Cellular Dynamics of Live Neurons and Oligodendrocytes. / Lim, Jong Min; Park, Chanjong; Park, Jin Sung; Kim, Changho; Chon, Bonghwan; Cho, Minhaeng.

In: Journal of Physical Chemistry Letters, 01.01.2019.

Research output: Contribution to journalArticle

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