Cytoprotective activity of elevated static pressure against oxidative stress in normal human fibroblasts

Sangnam Oh, Daeho Kwon, Eun Il Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

DNA damage in normal cells by reactive oxygen species can trigger cellular senescence and tumorigenesis. Therefore, many strategies have been developed to inhibit DNA damage and identify molecules that can prevent various oxidative stress-related diseases, including cancer. To investigate the effect of pressure, preconditioning with two types of atmospheric pressure (hyperbaric mild hyperoxia compressed with air (EP) and hyperbaric normoxia with nitrogen gas (HN)) was applied to normal human lung fibrob-lasts (WI-38) for 1 or 3 days, and then cells were treated with hydrogen peroxide to induce oxidative damage. Here, we have elucidated two types of pressure that can protect WI-38 cells from oxidative stress-induced DNA damage. To profile the expression of genes that contribute to the cytoprotective effects of the pressures, microarray analyses were performed. Extracellular matrix-, cell adhesion-, and cell cycle-related genes were highly involved in the cytoprotective effects of the pressures. Among the significantly expressed genes, fibronectin was the most inducible in WI-38 cells under the cytoprotective conditions against oxidative damage. Additionally, cellular fibronectin, and not the secreted isoform, acted as a cytoprotective marker at these pressures. Together, these findings suggest that elevated static pressures exhibit cytoprotective activity against oxidative stress-induced DNA damage by inducing cellular fibronectin.

Original languageEnglish
Pages (from-to)323-332
Number of pages10
JournalMolecular and Cellular Toxicology
Volume7
Issue number3
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

Oxidative stress
Fibroblasts
Oxidative Stress
Pressure
DNA Damage
Fibronectins
Genes
DNA
Compressed Air
cdc Genes
Hyperoxia
Atmospheric Pressure
Cell Aging
Cell adhesion
Microarray Analysis
Microarrays
Transcriptome
Cell Adhesion
Hydrogen Peroxide
Atmospheric pressure

Keywords

  • Cytoprotection
  • Elevated static pressure
  • Fibronectin

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Pathology and Forensic Medicine

Cite this

Cytoprotective activity of elevated static pressure against oxidative stress in normal human fibroblasts. / Oh, Sangnam; Kwon, Daeho; Lee, Eun Il.

In: Molecular and Cellular Toxicology, Vol. 7, No. 3, 01.12.2011, p. 323-332.

Research output: Contribution to journalArticle

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