Cytotoxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin on primary cultures of adult rat hepatocytes

K. H. Yang, Eui Ju Choi, S. Y. Choe

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Abstract

Primary cultures of adult rat hepatocytes were used to study the cytotoxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were treated with a single oral dose of 5, 10 or 25 Μg/kg of TCDD. At 2, 6, 10, 20, 30 days post-treatment, hepatocytes were isolated by a collagenase perfusion technique and maintained 48 hr as monolayers in serum-free medium in collagen-coated culture dishes. Uptake of ouabain was depressed in hepatocyte cultures isolated from all three doses of TCDD-treated rats. The effect was detected two days after TCDD-treatment and continued up to 30 days. The magnitude of the depression was directly related to the dose of TCDD. Hepatocyte cultures from TCDD-treated rats also showed depression in hormonally induced uptake of α-aminoisobutyric acid (AIB) and tyrosine aminotransferase (TAT) activity at 10 and 25 Μg/kg but not at 5 Μg/kg. These cytotoxic effects of TCDD could be demonstrated only when TCDD was given to rats prior to isolation of hepatocytes; no toxic effects were observed when TCDD was added directly to the hepatocyte cultures. Pretreatment of rats with 1,3,6,8-tetrachlorodibenzo-p-dioxin had no effect on ouabain uptake, AIB transport, or TAT activities.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalArchives of Environmental Contamination and Toxicology
Volume12
Issue number2
DOIs
Publication statusPublished - 1983 Mar 1
Externally publishedYes

Fingerprint

Cytotoxicity
dioxin
Rats
Hepatocytes
Tyrosine Transaminase
Aminoisobutyric Acids
Ouabain
Acids
Collagen
1,4-dioxin
Polychlorinated Dibenzodioxins
cytotoxicity
Monolayers
Poisons
Serum-Free Culture Media
Collagenases
collagen
acid
serum
Perfusion

ASJC Scopus subject areas

  • Toxicology
  • Environmental Chemistry
  • Environmental Science(all)

Cite this

Cytotoxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin on primary cultures of adult rat hepatocytes. / Yang, K. H.; Choi, Eui Ju; Choe, S. Y.

In: Archives of Environmental Contamination and Toxicology, Vol. 12, No. 2, 01.03.1983, p. 183-188.

Research output: Contribution to journalArticle

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