Dapagliflozin attenuates diabetes-induced diastolic dysfunction and cardiac fibrosis by regulating SGK1 signaling

Seul Gee Lee, Darae Kim, Jung Jae Lee, Hyun Ju Lee, Ro kyung Moon, Yong Joon Lee, Seung Jun Lee, Oh Hyun Lee, Choongki Kim, Jaewon Oh, Chan Joo Lee, Yong ho Lee, Seil Park, Ok Hee Jeon, Donghoon Choi, Geu Ru Hong, Jung Sun Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Recent studies have reported improved diastolic function in patients administered sodium-glucose cotransporter 2 inhibitors (SGLT2i). We aimed to investigate the effect of dapagliflozin on left ventricular (LV) diastolic function in a diabetic animal model and to determine the molecular and cellular mechanisms underlying its function. Methods: A total of 30 male New Zealand white rabbits were randomized into control, diabetes, or diabetes+dapagliflozin groups (n = 10/per each group). Diabetes was induced by intravenous alloxan. Cardiac function was evaluated using echocardiography. Myocardial samples were obtained for histologic and molecular evaluation. For cellular evaluation, fibrosis-induced cardiomyoblast (H9C2) cells were obtained, and transfection was performed for mechanism analysis (serum and glucocorticoid-regulated kinase 1 (SGK1) signaling analysis). Results: The diabetes+dapagliflozin group showed attenuation of diastolic dysfunction compared with the diabetes group. Dapagliflozin inhibited myocardial fibrosis via inhibition of SGK1 and epithelial sodium channel (ENaC) protein, which was observed both in myocardial tissue and H9C2 cells. In addition, dapagliflozin showed an anti-inflammatory effect and ameliorated mitochondrial disruption. Inhibition of SGK1 expression by siRNA decreased and ENaC and Na+/H+ exchanger isoform 1 (NHE1) expression was confirmed as significantly reduced as siSGK1 in the diabetes+dapagliflozin group. Conclusions: Dapagliflozin attenuated left ventricular diastolic dysfunction and cardiac fibrosis via regulation of SGK1 signaling. Dapagliflozin also reduced macrophages and inflammatory proteins and ameliorated mitochondrial disruption.

Original languageEnglish
Article number309
JournalBMC Medicine
Volume20
Issue number1
DOIs
Publication statusPublished - 2022 Dec

Keywords

  • Diabetes mellitus
  • Heart failure
  • Left ventricular diastolic function
  • Sodium-glucose cotransporter 2 inhibitor

ASJC Scopus subject areas

  • Medicine(all)

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