Dark fermentation of hydrogen from waste glycerol using hyperthermophilic eubacterium Thermotoga neapolitana

Tien Anh Ngo, Sang Jun Sim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Waste glycerol from bio-diesel manufacturing is currently held to be a new, attractive, and abundant resource for future bio-fuel. Bio-hydrogen production via the fermentation route was examined for the first time from pure glycerol and discharged waste glycerol as the sole substrate by the hyperthermophilic eubacterium Thermotoga neapolitana. The bacterium, directly fermenting pure glycerol and crude waste glycerol in the medium, showed respective retarded growth and low H 2 production 447 ± 22 mL H 2 L -1 and 437 ± 21 mL H 2 L -1. Attempting to improve the growth and H 2 production, small amount (1.5 g L -1) of itaconic acid was added into the culture medium. In this case, H 2 production from fermentation was approximately 620 ± 30 mL H 2 L -1 for both pure glycerol and pre-treated waste glycerol as main substrate, an increase of 36.6% compared with cultures grown without itaconic acid, respectively. Further optimization of the glycerol concentration and culture conditions revealed enhancements in growth, H 2 production, and glycerol utilization. The H 2 fermentation from waste glycerol is of potential importance as wastes from bio-diesel processes can be utilized to produce bio-hydrogen.

Original languageEnglish
Pages (from-to)466-473
Number of pages8
JournalEnvironmental Progress and Sustainable Energy
Volume31
Issue number3
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

Glycerol
Fermentation
fermentation
Hydrogen
hydrogen
diesel
substrate
Biodiesel
acid
biofuel
manufacturing
Acids
Substrates
Hydrogen production
bacterium
Culture Media
resource
Bacteria

Keywords

  • bio-diesel
  • glycerol
  • hydrogen production
  • Thermotaga neapolitana

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Environmental Engineering
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment
  • Environmental Science(all)

Cite this

Dark fermentation of hydrogen from waste glycerol using hyperthermophilic eubacterium Thermotoga neapolitana. / Ngo, Tien Anh; Sim, Sang Jun.

In: Environmental Progress and Sustainable Energy, Vol. 31, No. 3, 01.10.2012, p. 466-473.

Research output: Contribution to journalArticle

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