Dark quenched matrix metalloproteinase fluorogenic probe for imaging osteoarthritis development in vivo

Seulki Lee, Kyeongsoon Park, Seung Young Lee, Hee Ryu Ju, Jong Woong Park, Jun Ahn Hyung, Ick Chan Kwon, In Chan Youn, Kwang Meyung Kim, Kuiwon Choi

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The early detection of osteoarthritis (OA) is currently a key challenge in the field of rheumatology. Biochemical studies of OA have indicated that matrix metalloproteinase-13 (MMP-13) plays a central role in cartilage degradation. In this study, we describe the potential use of a dark-quenched fluorogenic MMP-13 probe to image MMP-13 in both in vitro and rat models. The imaging technique involved using a MMP-13 peptide substrate, near-infrared (NIR) dye, and a NIR dark quencher. The results from this study demonstrate that the use of a dark-quenched fluorogenic probe allows for the visual detection of MMP-13 in vitro and in OA-induced rat models. In particular, by targeting this OA biomarker, the symptoms of the early and late stages of OA can be readily monitored, imaged, and analyzed in a rapid and efficient fashion. We anticipate that this simple and highly efficient fluorogenic probe will assist in the clinical management of patients with OA, not only for early diagnosis but also to assess individual patient responses to new drug treatments.

Original languageEnglish
Pages (from-to)1743-1747
Number of pages5
JournalBioconjugate Chemistry
Volume19
Issue number9
DOIs
Publication statusPublished - 2008 Sep 1

Fingerprint

Matrix Metalloproteinase 13
Matrix Metalloproteinases
Osteoarthritis
Imaging techniques
Rats
Infrared radiation
Drug therapy
Cartilage
Biomarkers
Peptides
Rheumatology
Coloring Agents
Dyes
Metalloproteases
Early Diagnosis
Degradation
Substrates
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Dark quenched matrix metalloproteinase fluorogenic probe for imaging osteoarthritis development in vivo. / Lee, Seulki; Park, Kyeongsoon; Lee, Seung Young; Ju, Hee Ryu; Park, Jong Woong; Hyung, Jun Ahn; Kwon, Ick Chan; Youn, In Chan; Kim, Kwang Meyung; Choi, Kuiwon.

In: Bioconjugate Chemistry, Vol. 19, No. 9, 01.09.2008, p. 1743-1747.

Research output: Contribution to journalArticle

Lee, Seulki ; Park, Kyeongsoon ; Lee, Seung Young ; Ju, Hee Ryu ; Park, Jong Woong ; Hyung, Jun Ahn ; Kwon, Ick Chan ; Youn, In Chan ; Kim, Kwang Meyung ; Choi, Kuiwon. / Dark quenched matrix metalloproteinase fluorogenic probe for imaging osteoarthritis development in vivo. In: Bioconjugate Chemistry. 2008 ; Vol. 19, No. 9. pp. 1743-1747.
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