Death effector domain of a mammalian apoptosis mediator, FADD, induces bacterial cell death

Sang Won Lee, Young Gyu Ko, Sook Hee Bang, Key Sun Kim, Sunghoon Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

FADD is a mammalian pro-apoptotic mediator consisting of the N-terminal death effector domain (DED) and the C-terminal death domain (DD). The N-terminal 88-residue fragment of murine FADD was defined as the stable structural unit of DED, as determined by proteolytic digestion and conformational analysis. This domain induced bacterial as well as mammalian cell death, whereas the full-length or DD of FADD did not. The Escherichia coli cells expressing FADD-DED showed elongated cell morphology and an increased level of nicked chromosomal DNA and mutation. We lethality of FADD-DED was abolished by co-expression of thioredoxin and superoxide dismutase or relieved by the addition of vitamin E as a reducing agent and under anaerobic growth conditions. The toxicity of FADD-DED was genetically suppressed by various oxidoreductases of E coli. All these results suggest that the death effector domain of mammalian FADD induced bacterial cell death by enhancing cellular levels of reactive oxygen species (ROS).

Original languageEnglish
Pages (from-to)1540-1549
Number of pages10
JournalMolecular Microbiology
Volume35
Issue number6
DOIs
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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