Decontamination method using heat and relative humidity for radish seeds achieves a 7-log reduction of Escherichia coli O157: H7 without affecting product quality

Y. B. Kim, H. W. Kim, M. K. Song, Min-Suk Rhee

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4 Citations (Scopus)

Abstract

We developed a novel decontamination method to inactivate Escherichia coli O157:H7 on radish seeds without adversely affecting seed germination or product quality. The use of heat (55, 60, and 65 °C) combined with relative humidity (RH; 25, 45, 65, 85, and 100%) for 24. h was evaluated for effective microbial reduction and preservation of seed germination rates. A significant two-way interaction of heat and RH was observed for both microbial reduction and germination rate (P < 0.0001). Increases in heat and RH were associated with corresponding reductions in E. coli O157:H7 and in germination rate (P < 0.05). The order of lethality for the different treatments was generally as follows: no treatment < 55 °C/25-65% RH = 60 °C/25-45% RH = 65 °C/25% RH < 55 °C/85% RH = 60 °C/65% RH < 55 °C/100% RH = 60 °C/85-100% RH = 65 °C/45-100% RH. The most effective condition, 65 °C/45% RH, completely inactivated E. coli O157:H7 on the seeds (7.0. log. CFU/g reduction) and had no significant effect on the germination rate (85.4%; P > 0.05) or product quality. The method uses only heat and relative humidity without chemicals, and is thus applicable as a general decontamination procedure in spout producing plants where the use of growth chambers is the norm.

Original languageEnglish
Pages (from-to)42-46
Number of pages5
JournalInternational Journal of Food Microbiology
Volume201
DOIs
Publication statusPublished - 2015 May 8

Fingerprint

Raphanus
Escherichia coli O157
Decontamination
decontamination
radishes
Germination
Humidity
product quality
Escherichia coli
Seed
Seeds
Atmospheric humidity
relative humidity
Hot Temperature
heat
seed germination
seeds
seed products
growth chambers
methodology

Keywords

  • Escherichia coli O157:H7
  • Heat
  • Radish seeds
  • Relative humidity (RH)
  • Seed decontamination

ASJC Scopus subject areas

  • Food Science
  • Microbiology
  • Safety, Risk, Reliability and Quality

Cite this

@article{af52c792bf49431891168877f8c5752c,
title = "Decontamination method using heat and relative humidity for radish seeds achieves a 7-log reduction of Escherichia coli O157: H7 without affecting product quality",
abstract = "We developed a novel decontamination method to inactivate Escherichia coli O157:H7 on radish seeds without adversely affecting seed germination or product quality. The use of heat (55, 60, and 65 °C) combined with relative humidity (RH; 25, 45, 65, 85, and 100{\%}) for 24. h was evaluated for effective microbial reduction and preservation of seed germination rates. A significant two-way interaction of heat and RH was observed for both microbial reduction and germination rate (P < 0.0001). Increases in heat and RH were associated with corresponding reductions in E. coli O157:H7 and in germination rate (P < 0.05). The order of lethality for the different treatments was generally as follows: no treatment < 55 °C/25-65{\%} RH = 60 °C/25-45{\%} RH = 65 °C/25{\%} RH < 55 °C/85{\%} RH = 60 °C/65{\%} RH < 55 °C/100{\%} RH = 60 °C/85-100{\%} RH = 65 °C/45-100{\%} RH. The most effective condition, 65 °C/45{\%} RH, completely inactivated E. coli O157:H7 on the seeds (7.0. log. CFU/g reduction) and had no significant effect on the germination rate (85.4{\%}; P > 0.05) or product quality. The method uses only heat and relative humidity without chemicals, and is thus applicable as a general decontamination procedure in spout producing plants where the use of growth chambers is the norm.",
keywords = "Escherichia coli O157:H7, Heat, Radish seeds, Relative humidity (RH), Seed decontamination",
author = "Kim, {Y. B.} and Kim, {H. W.} and Song, {M. K.} and Min-Suk Rhee",
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T1 - Decontamination method using heat and relative humidity for radish seeds achieves a 7-log reduction of Escherichia coli O157

T2 - H7 without affecting product quality

AU - Kim, Y. B.

AU - Kim, H. W.

AU - Song, M. K.

AU - Rhee, Min-Suk

PY - 2015/5/8

Y1 - 2015/5/8

N2 - We developed a novel decontamination method to inactivate Escherichia coli O157:H7 on radish seeds without adversely affecting seed germination or product quality. The use of heat (55, 60, and 65 °C) combined with relative humidity (RH; 25, 45, 65, 85, and 100%) for 24. h was evaluated for effective microbial reduction and preservation of seed germination rates. A significant two-way interaction of heat and RH was observed for both microbial reduction and germination rate (P < 0.0001). Increases in heat and RH were associated with corresponding reductions in E. coli O157:H7 and in germination rate (P < 0.05). The order of lethality for the different treatments was generally as follows: no treatment < 55 °C/25-65% RH = 60 °C/25-45% RH = 65 °C/25% RH < 55 °C/85% RH = 60 °C/65% RH < 55 °C/100% RH = 60 °C/85-100% RH = 65 °C/45-100% RH. The most effective condition, 65 °C/45% RH, completely inactivated E. coli O157:H7 on the seeds (7.0. log. CFU/g reduction) and had no significant effect on the germination rate (85.4%; P > 0.05) or product quality. The method uses only heat and relative humidity without chemicals, and is thus applicable as a general decontamination procedure in spout producing plants where the use of growth chambers is the norm.

AB - We developed a novel decontamination method to inactivate Escherichia coli O157:H7 on radish seeds without adversely affecting seed germination or product quality. The use of heat (55, 60, and 65 °C) combined with relative humidity (RH; 25, 45, 65, 85, and 100%) for 24. h was evaluated for effective microbial reduction and preservation of seed germination rates. A significant two-way interaction of heat and RH was observed for both microbial reduction and germination rate (P < 0.0001). Increases in heat and RH were associated with corresponding reductions in E. coli O157:H7 and in germination rate (P < 0.05). The order of lethality for the different treatments was generally as follows: no treatment < 55 °C/25-65% RH = 60 °C/25-45% RH = 65 °C/25% RH < 55 °C/85% RH = 60 °C/65% RH < 55 °C/100% RH = 60 °C/85-100% RH = 65 °C/45-100% RH. The most effective condition, 65 °C/45% RH, completely inactivated E. coli O157:H7 on the seeds (7.0. log. CFU/g reduction) and had no significant effect on the germination rate (85.4%; P > 0.05) or product quality. The method uses only heat and relative humidity without chemicals, and is thus applicable as a general decontamination procedure in spout producing plants where the use of growth chambers is the norm.

KW - Escherichia coli O157:H7

KW - Heat

KW - Radish seeds

KW - Relative humidity (RH)

KW - Seed decontamination

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