Decreased expression of DNA repair proteins Ku70 and Mre11 is associated with aging and may contribute to the cellular senescence

Yeun Jin Ju, Kee Ho Lee, Jeong Eun Park, Yong Su Yi, Mi Yong Yun, Yong Ho Ham, Tae Jin Kim, Mi Choi Hyun, Jung Han Gwi, Jong Hoon Lee, Juneyoung Lee, Seol Han Jong, Kyung-Mi Lee, Gil-Hong Park

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The gradual loss of telomeric DNA can contribute to replicative senescence and thus, having longer telomeric DNA is generally considered to provide a longer lifespan. Maintenance and stabilization of telomeric DNA is assisted by binding of multiple DNA-binding proteins, including those involved in double strand break (DSB) repair. We reasoned that declining DSB repair capacity and increased telomere shortening in aged individuals may be associated with decreased expression of DSB repair proteins capable of telomere binding. Our data presented here show that among the DSB repair proteins tested, only the expression of Ku70 and Mre11 showed statistically significant age-dependent changes in human lymphocytes. Furthermore, we found that expressions of Ku70 and Mre11 are statistically correlated, which indicate that the function of Ku70 and Mre11 may be related. All the other DSB repair proteins tested, Sir2, TRF1 and Ku80, did not show any significant differences upon aging. In line with these data, people who live in the regional community (longevity group), which was found to have statistically longer average life span than the rest area, shows higher level of Ku70 expression than those living in the neighboring control community. Taken together, our data show, for the first time, that Ku70 and Mre11 may represent new biomarkers for aging and further suggest that maintenance of higher expression of Ku70 and Mre11 may be responsible for keeping longer life span observed in the longevity group.

Original languageEnglish
Pages (from-to)686-693
Number of pages8
JournalExperimental and Molecular Medicine
Volume38
Issue number6
Publication statusPublished - 2006 Dec 31

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Keywords

  • Aging
  • Ku70
  • Longevity
  • Mre11a protein
  • Telomere
  • Telomere binding proteins

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Ju, Y. J., Lee, K. H., Park, J. E., Yi, Y. S., Yun, M. Y., Ham, Y. H., Kim, T. J., Hyun, M. C., Gwi, J. H., Lee, J. H., Lee, J., Jong, S. H., Lee, K-M., & Park, G-H. (2006). Decreased expression of DNA repair proteins Ku70 and Mre11 is associated with aging and may contribute to the cellular senescence. Experimental and Molecular Medicine, 38(6), 686-693.