Deer bone oil extract suppresses lipopolysaccharide-induced inflammatory responses in RAW264.7 cells

Hyeon Son Choi, Suji Im, Yooheon Park, Ki Bae Hong, Hyung Joo Suh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study was to investigate the effect of deer bone oil extract (DBOE) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells. DBOE was fractionated by liquid-liquid extraction to obtain two fractions: methanol fraction (DBO-M) and hexane fraction (DBO-H). TLC showed that DBO-M had relatively more hydrophilic lipid complexes, including unsaturated fatty acids, than DBOE and DBO-H. The relative compositions of tetradecenoyl carnitine, α-linoleic acid, and palmitoleic acid increased in the DBO-M fraction by 61, 38, and 32%, respectively, compared with DBOE. The concentration of sugar moieties was 3-fold higher in the DBO-M fraction than DBOE and DBO-H. DBO-M significantly decreased LPS-induced nitric oxide (NO) production in RAW264.7 cells in a dose-dependent manner. This DBO-M-me-diated decrease in NO production was due to downregulation of mRNA and protein levels of inducible nitric oxide synthase (iNOS). In addition, mRNA expression of pro-inflammatory mediators, such as cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-12β, was suppressed by DBO-M. Our data showed that DBO-M, which has relatively higher sugar content than DBOE and DBO-H, could play an important role in suppressing inflammatory responses by controlling pro-inflammatory cytokines and mediators.

Original languageEnglish
Pages (from-to)593-600
Number of pages8
JournalBiological and Pharmaceutical Bulletin
Volume39
Issue number4
Publication statusPublished - 2016 Apr 1

Keywords

  • Anti-inflammatory response
  • Deer bone oil extract (DBOE)
  • Inducible nitric oxide synthase (iNOS)
  • Nitric oxide (NO)
  • RAW264.7

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

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