Abstract
The aim of this study was to investigate the effect of deer bone oil extract (DBOE) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells. DBOE was fractionated by liquid-liquid extraction to obtain two fractions: methanol fraction (DBO-M) and hexane fraction (DBO-H). TLC showed that DBO-M had relatively more hydrophilic lipid complexes, including unsaturated fatty acids, than DBOE and DBO-H. The relative compositions of tetradecenoyl carnitine, α-linoleic acid, and palmitoleic acid increased in the DBO-M fraction by 61, 38, and 32%, respectively, compared with DBOE. The concentration of sugar moieties was 3-fold higher in the DBO-M fraction than DBOE and DBO-H. DBO-M significantly decreased LPS-induced nitric oxide (NO) production in RAW264.7 cells in a dose-dependent manner. This DBO-M-me-diated decrease in NO production was due to downregulation of mRNA and protein levels of inducible nitric oxide synthase (iNOS). In addition, mRNA expression of pro-inflammatory mediators, such as cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-12β, was suppressed by DBO-M. Our data showed that DBO-M, which has relatively higher sugar content than DBOE and DBO-H, could play an important role in suppressing inflammatory responses by controlling pro-inflammatory cytokines and mediators.
| Original language | English |
|---|---|
| Pages (from-to) | 593-600 |
| Number of pages | 8 |
| Journal | Biological and Pharmaceutical Bulletin |
| Volume | 39 |
| Issue number | 4 |
| Publication status | Published - 2016 Apr 1 |
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Keywords
- Anti-inflammatory response
- Deer bone oil extract (DBOE)
- Inducible nitric oxide synthase (iNOS)
- Nitric oxide (NO)
- RAW264.7
ASJC Scopus subject areas
- Pharmaceutical Science
- Pharmacology
Cite this
Deer bone oil extract suppresses lipopolysaccharide-induced inflammatory responses in RAW264.7 cells. / Choi, Hyeon Son; Im, Suji; Park, Yooheon; Hong, Ki Bae; Suh, Hyung Joo.
In: Biological and Pharmaceutical Bulletin, Vol. 39, No. 4, 01.04.2016, p. 593-600.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Deer bone oil extract suppresses lipopolysaccharide-induced inflammatory responses in RAW264.7 cells
AU - Choi, Hyeon Son
AU - Im, Suji
AU - Park, Yooheon
AU - Hong, Ki Bae
AU - Suh, Hyung Joo
PY - 2016/4/1
Y1 - 2016/4/1
N2 - The aim of this study was to investigate the effect of deer bone oil extract (DBOE) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells. DBOE was fractionated by liquid-liquid extraction to obtain two fractions: methanol fraction (DBO-M) and hexane fraction (DBO-H). TLC showed that DBO-M had relatively more hydrophilic lipid complexes, including unsaturated fatty acids, than DBOE and DBO-H. The relative compositions of tetradecenoyl carnitine, α-linoleic acid, and palmitoleic acid increased in the DBO-M fraction by 61, 38, and 32%, respectively, compared with DBOE. The concentration of sugar moieties was 3-fold higher in the DBO-M fraction than DBOE and DBO-H. DBO-M significantly decreased LPS-induced nitric oxide (NO) production in RAW264.7 cells in a dose-dependent manner. This DBO-M-me-diated decrease in NO production was due to downregulation of mRNA and protein levels of inducible nitric oxide synthase (iNOS). In addition, mRNA expression of pro-inflammatory mediators, such as cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-12β, was suppressed by DBO-M. Our data showed that DBO-M, which has relatively higher sugar content than DBOE and DBO-H, could play an important role in suppressing inflammatory responses by controlling pro-inflammatory cytokines and mediators.
AB - The aim of this study was to investigate the effect of deer bone oil extract (DBOE) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells. DBOE was fractionated by liquid-liquid extraction to obtain two fractions: methanol fraction (DBO-M) and hexane fraction (DBO-H). TLC showed that DBO-M had relatively more hydrophilic lipid complexes, including unsaturated fatty acids, than DBOE and DBO-H. The relative compositions of tetradecenoyl carnitine, α-linoleic acid, and palmitoleic acid increased in the DBO-M fraction by 61, 38, and 32%, respectively, compared with DBOE. The concentration of sugar moieties was 3-fold higher in the DBO-M fraction than DBOE and DBO-H. DBO-M significantly decreased LPS-induced nitric oxide (NO) production in RAW264.7 cells in a dose-dependent manner. This DBO-M-me-diated decrease in NO production was due to downregulation of mRNA and protein levels of inducible nitric oxide synthase (iNOS). In addition, mRNA expression of pro-inflammatory mediators, such as cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-12β, was suppressed by DBO-M. Our data showed that DBO-M, which has relatively higher sugar content than DBOE and DBO-H, could play an important role in suppressing inflammatory responses by controlling pro-inflammatory cytokines and mediators.
KW - Anti-inflammatory response
KW - Deer bone oil extract (DBOE)
KW - Inducible nitric oxide synthase (iNOS)
KW - Nitric oxide (NO)
KW - RAW264.7
UR - http://www.scopus.com/inward/record.url?scp=84964608605&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84964608605&partnerID=8YFLogxK
M3 - Article
C2 - 27040632
AN - SCOPUS:84964608605
VL - 39
SP - 593
EP - 600
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 4
ER -