Deer bone oil extract suppresses lipopolysaccharide-induced inflammatory responses in RAW264.7 cells

Hyeon Son Choi, Suji Im, Yooheon Park, Ki Bae Hong, Hyung Joo Suh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study was to investigate the effect of deer bone oil extract (DBOE) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells. DBOE was fractionated by liquid-liquid extraction to obtain two fractions: methanol fraction (DBO-M) and hexane fraction (DBO-H). TLC showed that DBO-M had relatively more hydrophilic lipid complexes, including unsaturated fatty acids, than DBOE and DBO-H. The relative compositions of tetradecenoyl carnitine, α-linoleic acid, and palmitoleic acid increased in the DBO-M fraction by 61, 38, and 32%, respectively, compared with DBOE. The concentration of sugar moieties was 3-fold higher in the DBO-M fraction than DBOE and DBO-H. DBO-M significantly decreased LPS-induced nitric oxide (NO) production in RAW264.7 cells in a dose-dependent manner. This DBO-M-me-diated decrease in NO production was due to downregulation of mRNA and protein levels of inducible nitric oxide synthase (iNOS). In addition, mRNA expression of pro-inflammatory mediators, such as cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-12β, was suppressed by DBO-M. Our data showed that DBO-M, which has relatively higher sugar content than DBOE and DBO-H, could play an important role in suppressing inflammatory responses by controlling pro-inflammatory cytokines and mediators.

Original languageEnglish
Pages (from-to)593-600
Number of pages8
JournalBiological and Pharmaceutical Bulletin
Volume39
Issue number4
Publication statusPublished - 2016 Apr 1

Fingerprint

Deer
Lipopolysaccharides
Oils
Bone and Bones
Hexanes
Nitric Oxide
Messenger RNA
Liquid-Liquid Extraction
Carnitine
Nitric Oxide Synthase Type II
Linoleic Acid
Cyclooxygenase 2
Interleukin-12
Unsaturated Fatty Acids
Interleukin-1
Methanol
Down-Regulation
Cytokines
Lipids
Proteins

Keywords

  • Anti-inflammatory response
  • Deer bone oil extract (DBOE)
  • Inducible nitric oxide synthase (iNOS)
  • Nitric oxide (NO)
  • RAW264.7

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Deer bone oil extract suppresses lipopolysaccharide-induced inflammatory responses in RAW264.7 cells. / Choi, Hyeon Son; Im, Suji; Park, Yooheon; Hong, Ki Bae; Suh, Hyung Joo.

In: Biological and Pharmaceutical Bulletin, Vol. 39, No. 4, 01.04.2016, p. 593-600.

Research output: Contribution to journalArticle

Choi, Hyeon Son ; Im, Suji ; Park, Yooheon ; Hong, Ki Bae ; Suh, Hyung Joo. / Deer bone oil extract suppresses lipopolysaccharide-induced inflammatory responses in RAW264.7 cells. In: Biological and Pharmaceutical Bulletin. 2016 ; Vol. 39, No. 4. pp. 593-600.
@article{cab65c46a68748a482f201590c14dd86,
title = "Deer bone oil extract suppresses lipopolysaccharide-induced inflammatory responses in RAW264.7 cells",
abstract = "The aim of this study was to investigate the effect of deer bone oil extract (DBOE) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells. DBOE was fractionated by liquid-liquid extraction to obtain two fractions: methanol fraction (DBO-M) and hexane fraction (DBO-H). TLC showed that DBO-M had relatively more hydrophilic lipid complexes, including unsaturated fatty acids, than DBOE and DBO-H. The relative compositions of tetradecenoyl carnitine, α-linoleic acid, and palmitoleic acid increased in the DBO-M fraction by 61, 38, and 32{\%}, respectively, compared with DBOE. The concentration of sugar moieties was 3-fold higher in the DBO-M fraction than DBOE and DBO-H. DBO-M significantly decreased LPS-induced nitric oxide (NO) production in RAW264.7 cells in a dose-dependent manner. This DBO-M-me-diated decrease in NO production was due to downregulation of mRNA and protein levels of inducible nitric oxide synthase (iNOS). In addition, mRNA expression of pro-inflammatory mediators, such as cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-12β, was suppressed by DBO-M. Our data showed that DBO-M, which has relatively higher sugar content than DBOE and DBO-H, could play an important role in suppressing inflammatory responses by controlling pro-inflammatory cytokines and mediators.",
keywords = "Anti-inflammatory response, Deer bone oil extract (DBOE), Inducible nitric oxide synthase (iNOS), Nitric oxide (NO), RAW264.7",
author = "Choi, {Hyeon Son} and Suji Im and Yooheon Park and Hong, {Ki Bae} and Suh, {Hyung Joo}",
year = "2016",
month = "4",
day = "1",
language = "English",
volume = "39",
pages = "593--600",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "4",

}

TY - JOUR

T1 - Deer bone oil extract suppresses lipopolysaccharide-induced inflammatory responses in RAW264.7 cells

AU - Choi, Hyeon Son

AU - Im, Suji

AU - Park, Yooheon

AU - Hong, Ki Bae

AU - Suh, Hyung Joo

PY - 2016/4/1

Y1 - 2016/4/1

N2 - The aim of this study was to investigate the effect of deer bone oil extract (DBOE) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells. DBOE was fractionated by liquid-liquid extraction to obtain two fractions: methanol fraction (DBO-M) and hexane fraction (DBO-H). TLC showed that DBO-M had relatively more hydrophilic lipid complexes, including unsaturated fatty acids, than DBOE and DBO-H. The relative compositions of tetradecenoyl carnitine, α-linoleic acid, and palmitoleic acid increased in the DBO-M fraction by 61, 38, and 32%, respectively, compared with DBOE. The concentration of sugar moieties was 3-fold higher in the DBO-M fraction than DBOE and DBO-H. DBO-M significantly decreased LPS-induced nitric oxide (NO) production in RAW264.7 cells in a dose-dependent manner. This DBO-M-me-diated decrease in NO production was due to downregulation of mRNA and protein levels of inducible nitric oxide synthase (iNOS). In addition, mRNA expression of pro-inflammatory mediators, such as cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-12β, was suppressed by DBO-M. Our data showed that DBO-M, which has relatively higher sugar content than DBOE and DBO-H, could play an important role in suppressing inflammatory responses by controlling pro-inflammatory cytokines and mediators.

AB - The aim of this study was to investigate the effect of deer bone oil extract (DBOE) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells. DBOE was fractionated by liquid-liquid extraction to obtain two fractions: methanol fraction (DBO-M) and hexane fraction (DBO-H). TLC showed that DBO-M had relatively more hydrophilic lipid complexes, including unsaturated fatty acids, than DBOE and DBO-H. The relative compositions of tetradecenoyl carnitine, α-linoleic acid, and palmitoleic acid increased in the DBO-M fraction by 61, 38, and 32%, respectively, compared with DBOE. The concentration of sugar moieties was 3-fold higher in the DBO-M fraction than DBOE and DBO-H. DBO-M significantly decreased LPS-induced nitric oxide (NO) production in RAW264.7 cells in a dose-dependent manner. This DBO-M-me-diated decrease in NO production was due to downregulation of mRNA and protein levels of inducible nitric oxide synthase (iNOS). In addition, mRNA expression of pro-inflammatory mediators, such as cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-12β, was suppressed by DBO-M. Our data showed that DBO-M, which has relatively higher sugar content than DBOE and DBO-H, could play an important role in suppressing inflammatory responses by controlling pro-inflammatory cytokines and mediators.

KW - Anti-inflammatory response

KW - Deer bone oil extract (DBOE)

KW - Inducible nitric oxide synthase (iNOS)

KW - Nitric oxide (NO)

KW - RAW264.7

UR - http://www.scopus.com/inward/record.url?scp=84964608605&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964608605&partnerID=8YFLogxK

M3 - Article

C2 - 27040632

AN - SCOPUS:84964608605

VL - 39

SP - 593

EP - 600

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 4

ER -