Deformable l-shaped microwell array for trapping pairs of heterogeneous cells

G. H. Lee; S. H. Kim; A. R. Kang; S. Takayama; Sang Hoon Lee; J. Y. Park

Deformable l-shaped microwell array for trapping pairs of heterogeneous cells

G. H. Lee, S. H. Kim, A. R. Kang, S. Takayama, Sang Hoon Lee, J. Y. Park

Department of Bio-convergence Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We propose an L-shaped microwell (L-microwell) array that relies on the elasticity of a polydimethylsiloxane (PDMS) substrate for trapping and pairing heterogeneous cells. We designed an L-microwell suitable for trapping single cell in each branch via stretching/releasing the PDMS substrate, and also performed 3D time-dependent diffusion simulations to visualize how cell secreted molecules diffuse in the L-microwell and communicate with the partner cell. We produced over 5000 cell pairs in one 2.25 cm² array with about 30 000 L-microwells. The proposed L-microwell array offers a versatile and convenient cell pairing method.

Original language	English
Title of host publication	MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	502-504
Number of pages	3
ISBN (Electronic)	9780979806483
Publication status	Published - 2015 Jan 1
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 2015 Oct 25 → 2015 Oct 29

Other

Other	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Country/Territory	Korea, Republic of
City	Gyeongju
Period	15/10/25 → 15/10/29

Keywords

Cell pairing
L-shaped microwell
Substrate deformation

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

Deformable l-shaped microwell array for trapping pairs of heterogeneous cells. / Lee, G. H.; Kim, S. H.; Kang, A. R.; Takayama, S.; Lee, Sang Hoon; Park, J. Y.

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 502-504.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lee, GH, Kim, SH, Kang, AR, Takayama, S, Lee, SH & Park, JY 2015, Deformable l-shaped microwell array for trapping pairs of heterogeneous cells. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 502-504, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 15/10/25.

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AU - Lee, G. H.

AU - Kim, S. H.

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AU - Lee, Sang Hoon

AU - Park, J. Y.

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N2 - We propose an L-shaped microwell (L-microwell) array that relies on the elasticity of a polydimethylsiloxane (PDMS) substrate for trapping and pairing heterogeneous cells. We designed an L-microwell suitable for trapping single cell in each branch via stretching/releasing the PDMS substrate, and also performed 3D time-dependent diffusion simulations to visualize how cell secreted molecules diffuse in the L-microwell and communicate with the partner cell. We produced over 5000 cell pairs in one 2.25 cm2 array with about 30 000 L-microwells. The proposed L-microwell array offers a versatile and convenient cell pairing method.

AB - We propose an L-shaped microwell (L-microwell) array that relies on the elasticity of a polydimethylsiloxane (PDMS) substrate for trapping and pairing heterogeneous cells. We designed an L-microwell suitable for trapping single cell in each branch via stretching/releasing the PDMS substrate, and also performed 3D time-dependent diffusion simulations to visualize how cell secreted molecules diffuse in the L-microwell and communicate with the partner cell. We produced over 5000 cell pairs in one 2.25 cm2 array with about 30 000 L-microwells. The proposed L-microwell array offers a versatile and convenient cell pairing method.

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KW - Substrate deformation

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Deformable l-shaped microwell array for trapping pairs of heterogeneous cells

Abstract

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Keywords

ASJC Scopus subject areas

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