Abstract
We propose an L-shaped microwell (L-microwell) array that relies on the elasticity of a polydimethylsiloxane (PDMS) substrate for trapping and pairing heterogeneous cells. We designed an L-microwell suitable for trapping single cell in each branch via stretching/releasing the PDMS substrate, and also performed 3D time-dependent diffusion simulations to visualize how cell secreted molecules diffuse in the L-microwell and communicate with the partner cell. We produced over 5000 cell pairs in one 2.25 cm2 array with about 30 000 L-microwells. The proposed L-microwell array offers a versatile and convenient cell pairing method.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 502-504 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| Publication status | Published - 2015 Jan 1 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 2015 Oct 25 → 2015 Oct 29 |
Other
| Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country | Korea, Republic of |
| City | Gyeongju |
| Period | 15/10/25 → 15/10/29 |
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Keywords
- Cell pairing
- L-shaped microwell
- Substrate deformation
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
Deformable l-shaped microwell array for trapping pairs of heterogeneous cells. / Lee, G. H.; Kim, S. H.; Kang, A. R.; Takayama, S.; Lee, Sang Hoon; Park, J. Y.
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 502-504.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Deformable l-shaped microwell array for trapping pairs of heterogeneous cells
AU - Lee, G. H.
AU - Kim, S. H.
AU - Kang, A. R.
AU - Takayama, S.
AU - Lee, Sang Hoon
AU - Park, J. Y.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - We propose an L-shaped microwell (L-microwell) array that relies on the elasticity of a polydimethylsiloxane (PDMS) substrate for trapping and pairing heterogeneous cells. We designed an L-microwell suitable for trapping single cell in each branch via stretching/releasing the PDMS substrate, and also performed 3D time-dependent diffusion simulations to visualize how cell secreted molecules diffuse in the L-microwell and communicate with the partner cell. We produced over 5000 cell pairs in one 2.25 cm2 array with about 30 000 L-microwells. The proposed L-microwell array offers a versatile and convenient cell pairing method.
AB - We propose an L-shaped microwell (L-microwell) array that relies on the elasticity of a polydimethylsiloxane (PDMS) substrate for trapping and pairing heterogeneous cells. We designed an L-microwell suitable for trapping single cell in each branch via stretching/releasing the PDMS substrate, and also performed 3D time-dependent diffusion simulations to visualize how cell secreted molecules diffuse in the L-microwell and communicate with the partner cell. We produced over 5000 cell pairs in one 2.25 cm2 array with about 30 000 L-microwells. The proposed L-microwell array offers a versatile and convenient cell pairing method.
KW - Cell pairing
KW - L-shaped microwell
KW - Substrate deformation
UR - http://www.scopus.com/inward/record.url?scp=84983247277&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84983247277&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84983247277
SP - 502
EP - 504
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
ER -