Deformable l-shaped microwell array for trapping pairs of heterogeneous cells

G. H. Lee, S. H. Kim, A. R. Kang, S. Takayama, Sang Hoon Lee, J. Y. Park

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We propose an L-shaped microwell (L-microwell) array that relies on the elasticity of a polydimethylsiloxane (PDMS) substrate for trapping and pairing heterogeneous cells. We designed an L-microwell suitable for trapping single cell in each branch via stretching/releasing the PDMS substrate, and also performed 3D time-dependent diffusion simulations to visualize how cell secreted molecules diffuse in the L-microwell and communicate with the partner cell. We produced over 5000 cell pairs in one 2.25 cm2 array with about 30 000 L-microwells. The proposed L-microwell array offers a versatile and convenient cell pairing method.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages502-504
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015 Jan 1
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Keywords

  • Cell pairing
  • L-shaped microwell
  • Substrate deformation

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Lee, G. H., Kim, S. H., Kang, A. R., Takayama, S., Lee, S. H., & Park, J. Y. (2015). Deformable l-shaped microwell array for trapping pairs of heterogeneous cells. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 502-504). Chemical and Biological Microsystems Society.