Abstract
We propose an L-shaped microwell (L-microwell) array that relies on the elasticity of a polydimethylsiloxane (PDMS) substrate for trapping and pairing heterogeneous cells. We designed an L-microwell suitable for trapping single cell in each branch via stretching/releasing the PDMS substrate, and also performed 3D time-dependent diffusion simulations to visualize how cell secreted molecules diffuse in the L-microwell and communicate with the partner cell. We produced over 5000 cell pairs in one 2.25 cm2 array with about 30 000 L-microwells. The proposed L-microwell array offers a versatile and convenient cell pairing method.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 502-504 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| Publication status | Published - 2015 Jan 1 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 2015 Oct 25 → 2015 Oct 29 |
Other
| Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country | Korea, Republic of |
| City | Gyeongju |
| Period | 15/10/25 → 15/10/29 |
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Keywords
- Cell pairing
- L-shaped microwell
- Substrate deformation
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
Lee, G. H., Kim, S. H., Kang, A. R., Takayama, S., Lee, S. H., & Park, J. Y. (2015). Deformable l-shaped microwell array for trapping pairs of heterogeneous cells. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 502-504). Chemical and Biological Microsystems Society.