Degradation of cellulose by the major endoglucanase produced from the brown-rot fungus Fomitopsis pinicola

Jeong Jun Yoon, Chang Jun Cha, Yeong Suk Kim, Wook Kim

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

An endoglucanase that is able to degrade both crystalline and amorphous cellulose was purified from the culture filtrates of the brown-rot fungus Fomitopsis pinicola grown on cellulose. An apparent molecular weight of the purified enzyme was ∼32 kDa by SDS-PAGE analysis. The enzyme was purified 11-fold with a specific activity of 944 U/mg protein against CMC. The partial amino acid sequences of the purified endoglucanase had high homology with endo-β-1,4-glucanase of glycosyl hydrolase family 5 from other fungi. The Km and Kcat values for CMC were 12 mg CMC/ml and 670/s, respectively. The purified EG hydrolyzed both cellotetraose (G4) and cellopentaose (G5), but did not degrade either cellobiose (G2) or cellotriose (G3).

Original languageEnglish
Pages (from-to)1373-1378
Number of pages6
JournalBiotechnology letters
Volume30
Issue number8
DOIs
Publication statusPublished - 2008 Aug

Keywords

  • Brown-rot fungus
  • Cellulose
  • Endoglucanase
  • Fomitopsis pinicola
  • Microcrystalline cellulose hydrolysis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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