Dehydroepiandrosterone inhibits the death of immunostimulated rat C6 glioma cells deprived of glucose

Chan Young Shin, Ji Woong Choi, Eun Sook Jang, Chung Ju, Won-Ki Kim, Hyoung Chun Kim, Chang R. Choi, Kwang Ho Ko

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Pretreatment of interferon-γ and lipopolysaccharides made C6 glioma cells highly vulnerable to glucose deprivation. Neither 12 h of glucose deprivation nor 2-day treatment with interferon-γ (100 U/ml) and lipopolysaccharides (1 μg/ml) altered the viability of C6 glioma cells. However, significant death of immunostimulated C6 glioma cells was observed after 5 h of glucose deprivation. The augmented death was prevented by dehydroepiandrosterone (DHEA) treatment during immunostimulation, but not by DHEA treatment during glucose deprivation. DHEA reduced the rise in nitrotyrosine immunoreactivity, a marker of peroxynitrite, and superoxide production in glucose-deprived immunostimulated C6 glioma cells. DHEA, however, did not protect glucose-deprived C6 glioma cells from the exogenously produced peroxynitrite by 3-morpholinosydnonimine. Further, DHEA did not alter the production of total reactive oxygen species and nitric oxide in immunostimulated C6 glioma cells. Superoxide dismutase (SOD) and the synthetic SOD mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin inhibited the death of glucose-deprived immunostimulated C6 glioma cells. In addition, a superoxide anion generator paraquat reversed the protective effect of DHEA on the augmented death. The data indicate that DHEA prevents the glucose deprivation-evoked augmented death by inhibiting the production of superoxide anion in immunostimulated C6 glioma cells.

Original languageEnglish
Pages (from-to)267-275
Number of pages9
JournalBrain Research
Volume922
Issue number2
DOIs
Publication statusPublished - 2001 Dec 20
Externally publishedYes

Fingerprint

Dehydroepiandrosterone
Glioma
Glucose
Superoxides
Peroxynitrous Acid
Interferons
Superoxide Dismutase
Lipopolysaccharides
Paraquat
Immunization
Reactive Oxygen Species
Nitric Oxide
Therapeutics

Keywords

  • C6 glioma
  • Dehydroepiandrosterone
  • Glucose deprivation
  • Immunostimulation
  • Peroxynitrite

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Dehydroepiandrosterone inhibits the death of immunostimulated rat C6 glioma cells deprived of glucose. / Young Shin, Chan; Choi, Ji Woong; Sook Jang, Eun; Ju, Chung; Kim, Won-Ki; Kim, Hyoung Chun; Choi, Chang R.; Ho Ko, Kwang.

In: Brain Research, Vol. 922, No. 2, 20.12.2001, p. 267-275.

Research output: Contribution to journalArticle

Young Shin, C, Choi, JW, Sook Jang, E, Ju, C, Kim, W-K, Kim, HC, Choi, CR & Ho Ko, K 2001, 'Dehydroepiandrosterone inhibits the death of immunostimulated rat C6 glioma cells deprived of glucose', Brain Research, vol. 922, no. 2, pp. 267-275. https://doi.org/10.1016/S0006-8993(01)03185-7
Young Shin, Chan ; Choi, Ji Woong ; Sook Jang, Eun ; Ju, Chung ; Kim, Won-Ki ; Kim, Hyoung Chun ; Choi, Chang R. ; Ho Ko, Kwang. / Dehydroepiandrosterone inhibits the death of immunostimulated rat C6 glioma cells deprived of glucose. In: Brain Research. 2001 ; Vol. 922, No. 2. pp. 267-275.
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