Deletion mutations conferring substrate spectrum extension in the class A β-lactamase

Junghyun Hwang, Kwang Hwi Cho, Han Song, Hyojeong Yi, Heenam Stanley Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We describe four new deletion mutations in a class A β-lactamase PenA in Burkholderia thailandensis, each conferring an extended substrate spectrum. Single-amino-acid deletions T171del, I173del, and P174del and a two-amino-acid deletion, R165-T167delinsP, occurred in the omega loop, increasing the flexibility of the binding cavity. This rare collection of mutations has significance, allowing exploration of the diverse evolutionary trajectories of β-lactamases and as potential future mutations conferring high-level ceftazidime resistance on isolates from clinical settings, compared with amino acid substitution mutations. Copyright

Original languageEnglish
Pages (from-to)6265-6269
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume58
Issue number10
DOIs
Publication statusPublished - 2014 Oct 1

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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