TY - JOUR
T1 - Deletion mutations conferring substrate spectrum extension in the class A β-lactamase
AU - Hwang, Junghyun
AU - Cho, Kwang Hwi
AU - Song, Han
AU - Yi, Hyojeong
AU - Kim, Heenam Stanley
N1 - Publisher Copyright:
© 2014, American Society for Microbiology. All Rights Reserved.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - We describe four new deletion mutations in a class A β-lactamase PenA in Burkholderia thailandensis, each conferring an extended substrate spectrum. Single-amino-acid deletions T171del, I173del, and P174del and a two-amino-acid deletion, R165-T167delinsP, occurred in the omega loop, increasing the flexibility of the binding cavity. This rare collection of mutations has significance, allowing exploration of the diverse evolutionary trajectories of β-lactamases and as potential future mutations conferring high-level ceftazidime resistance on isolates from clinical settings, compared with amino acid substitution mutations.
AB - We describe four new deletion mutations in a class A β-lactamase PenA in Burkholderia thailandensis, each conferring an extended substrate spectrum. Single-amino-acid deletions T171del, I173del, and P174del and a two-amino-acid deletion, R165-T167delinsP, occurred in the omega loop, increasing the flexibility of the binding cavity. This rare collection of mutations has significance, allowing exploration of the diverse evolutionary trajectories of β-lactamases and as potential future mutations conferring high-level ceftazidime resistance on isolates from clinical settings, compared with amino acid substitution mutations.
UR - http://www.scopus.com/inward/record.url?scp=84907262637&partnerID=8YFLogxK
U2 - 10.1128/AAC.02648-14
DO - 10.1128/AAC.02648-14
M3 - Article
C2 - 25049254
AN - SCOPUS:84907262637
SN - 0066-4804
VL - 58
SP - 6265
EP - 6269
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 10
ER -
