Deletion mutations conferring substrate spectrum extension in the class A β-lactamase

Junghyun Hwang, Kwang Hwi Cho, Han Song, Hyojeong Yi, Heenam Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We describe four new deletion mutations in a class A β-lactamase PenA in Burkholderia thailandensis, each conferring an extended substrate spectrum. Single-amino-acid deletions T171del, I173del, and P174del and a two-amino-acid deletion, R165-T167delinsP, occurred in the omega loop, increasing the flexibility of the binding cavity. This rare collection of mutations has significance, allowing exploration of the diverse evolutionary trajectories of β-lactamases and as potential future mutations conferring high-level ceftazidime resistance on isolates from clinical settings, compared with amino acid substitution mutations. Copyright

Original languageEnglish
Pages (from-to)6265-6269
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume58
Issue number10
DOIs
Publication statusPublished - 2014 Oct 1

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Sequence Deletion
Mutation
Burkholderia
Amino Acids
Ceftazidime
Amino Acid Substitution

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Deletion mutations conferring substrate spectrum extension in the class A β-lactamase. / Hwang, Junghyun; Cho, Kwang Hwi; Song, Han; Yi, Hyojeong; Kim, Heenam.

In: Antimicrobial Agents and Chemotherapy, Vol. 58, No. 10, 01.10.2014, p. 6265-6269.

Research output: Contribution to journalArticle

Hwang, Junghyun ; Cho, Kwang Hwi ; Song, Han ; Yi, Hyojeong ; Kim, Heenam. / Deletion mutations conferring substrate spectrum extension in the class A β-lactamase. In: Antimicrobial Agents and Chemotherapy. 2014 ; Vol. 58, No. 10. pp. 6265-6269.
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