Deletion of PHO13 improves aerobic l-arabinose fermentation in engineered Saccharomyces cerevisiae

Suji Ye, Deokyeol Jeong, Jong Cheol Shon, Kwang Hyeon Liu, Kyoung Heon Kim, Minhye Shin, Soo Rin Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Pentose sugars are increasingly being used in industrial applications of Saccharomyces cerevisiae. Although l-arabinose is a highlighted pentose that has been identified as next-generation biomass, arabinose fermentation has not yet undergone extensive development for industrial utilization. In this study, we integrated a heterologous fungal arabinose pathway with a deletion of PHO13 phosphatase gene. PHO13 deletion increased arabinose consumption rate and specific ethanol productivity under aerobic conditions and consequently depleted sedoheptulose by activation of the TAL1 gene. Global metabolite profiling indicated upregulation of the pentose phosphate pathway and downstream effects such as trehalose accumulation and downregulation of the TCA cycle. Our results suggest that engineering of PHO13 has ample potential for arabinose conversion to ethanol as an industrial source for biofuels.

Original languageEnglish
JournalJournal of Industrial Microbiology and Biotechnology
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Arabinose
Yeast
Fermentation
Saccharomyces cerevisiae
Ethanol
Pentoses
Genes
Phosphatases
Biofuels
Metabolites
Sugars
Industrial applications
Phosphates
Biomass
Productivity
Chemical activation
Pentose Phosphate Pathway
Trehalose
Phosphoric Monoester Hydrolases
Transcriptional Activation

Keywords

  • l-arabinose
  • PHO13
  • Sedoheptulose
  • TAL1

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Deletion of PHO13 improves aerobic l-arabinose fermentation in engineered Saccharomyces cerevisiae. / Ye, Suji; Jeong, Deokyeol; Shon, Jong Cheol; Liu, Kwang Hyeon; Kim, Kyoung Heon; Shin, Minhye; Kim, Soo Rin.

In: Journal of Industrial Microbiology and Biotechnology, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Kim, Soo Rin

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