Deletion of PHO13 improves aerobic l-arabinose fermentation in engineered Saccharomyces cerevisiae

Suji Ye, Deokyeol Jeong, Jong Cheol Shon, Kwang Hyeon Liu, Kyoung Heon Kim, Minhye Shin, Soo Rin Kim

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Pentose sugars are increasingly being used in industrial applications of Saccharomyces cerevisiae. Although l-arabinose is a highlighted pentose that has been identified as next-generation biomass, arabinose fermentation has not yet undergone extensive development for industrial utilization. In this study, we integrated a heterologous fungal arabinose pathway with a deletion of PHO13 phosphatase gene. PHO13 deletion increased arabinose consumption rate and specific ethanol productivity under aerobic conditions and consequently depleted sedoheptulose by activation of the TAL1 gene. Global metabolite profiling indicated upregulation of the pentose phosphate pathway and downstream effects such as trehalose accumulation and downregulation of the TCA cycle. Our results suggest that engineering of PHO13 has ample potential for arabinose conversion to ethanol as an industrial source for biofuels.

Original languageEnglish
Pages (from-to)1725-1731
Number of pages7
JournalJournal of Industrial Microbiology and Biotechnology
Volume46
Issue number12
DOIs
Publication statusPublished - 2019 Dec 1

Keywords

  • PHO13
  • Sedoheptulose
  • TAL1
  • l-arabinose

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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