Demethylation of RUNX3 by vincristine in colorectal adenocarcinoma cells

Ji Wook Moon, Soo Kyung Lee, Jung Ok Lee, Ji Hae Kim, Nami Kim, Jin Kim, Hyeon Soo Kim, Sun Hwa Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Methylation-mediated inactivation of tumor-suppressor genes is a critical event during the pathogenesis of many malignancies. Vincristine is a conventional anticancer drug used to treat various types of cancers. However, few studies describe the epigenetic-based effects of vincristine. In this study, changes in the methylation of runt-related transcription factor-3 (RUNX3) were investigated in CCD18Co normal colon cells and DLD-1 colorectal adenocarcinoma cells. Materials and Methods: CCD18Co and DLD-1 cells were treated with vincristine, and the methylation status was assessed using quantitative methylation-specific polymerase chain reaction (QMSP). Eleven normal colon tissues and 105 colorectal cancer tissues were investigated by methylation and mRNA expression of RUNX3 using QMSP and real-time reverse transcription polymerase chain reaction (real time-PCR). Results: RUNX3 was demethylated after vincristine treatment in DLD-1 cells. The expression of RUNX3 mRNA was down-regulated in DLD-1 cells because of DNA hypermethylation, but was restored after vincristine treatment. In addition, hypermethylation of RUNX3 was detected in 70 out of 105 colorectal carcinomas (66.7%). RUNX3 hypermethylation was greater in colon cancer tissues than in rectal cancer tissues. The expression of RUNX3 mRNA was reduced in 68 out of 105 colorectal cancer tissues (64.8%). Conclusion: These results demonstrate that vincristine demethylates RUNX3 in colorectal adenocarcinoma cells, and restores its expression.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalAnticancer research
Volume34
Issue number1
Publication statusPublished - 2014 Jan 1

Keywords

  • Colonic neoplasms
  • Demethylation
  • DNA methylation
  • Methylation-specific polymerase chain reaction
  • RUNX3
  • Vincristine

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Moon, J. W., Lee, S. K., Lee, J. O., Kim, J. H., Kim, N., Kim, J., Kim, H. S., & Park, S. H. (2014). Demethylation of RUNX3 by vincristine in colorectal adenocarcinoma cells. Anticancer research, 34(1), 133-140.