Density-dependent modulation of vascular smooth muscle α-actin biosynthetic processing in differentiated BC3H1 myogenic cells

A. R. Strauch, Bon Hong Min, J. C. Reeser, H. Yan, D. N. Foster, M. D. Berman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The expression of vascular smooth muscle (VSM) α-actin mRNA during BC3H1 myogenic cell differentiation is specifically stimulated by conditions of high cell density. Non-proteolytic dissociation of cell-cell and cell-matrix contacts in post-confluent cultures of BC3H1 myocytes using EDTA promotes loss of the differentiated morphological phenotype. EDTA-dispersed myocytes exhibit an undifferentiated fibroblastoid appearance and contained reduced levels of both VSM and skeletal α-actin mRNA. Muscle α-actin mRNA levels in EDTA-dispersed myocytes were not restored to that observed in confluent myocyte preparations by experimental manipulation of cell density conditions. Pulse-labeling techniques using L-[35S]cysteine to identify muscle actin biosynthetic intermediates revealed that EDTA-dispersed myocytes expressed nascent forms of both the VSM and skeletal muscle α-actin polypeptide chains. However EDTA-dispersed myocytes were less efficient in the post-translational processing of immature VSM α-actin compared to non-dispersed myocytes. Simple cell-to-cell contact may mediate VSM α-actin processing efficiency since high-density preparations of EDTA-dispersed myocytes processed more VSM α-actin intermediate than myocytes plated at low density. The actin isoform selectivity of the response to modulation of intercellular contacts suggests that actin biosynthesis in BC3H1 myogenic cells involves mechanisms capable of discriminating between different isoform classes of nascent actin polypeptide chains.

Original languageEnglish
Pages (from-to)266-278
Number of pages13
JournalJournal of Cellular Biochemistry
Volume50
Issue number3
DOIs
Publication statusPublished - 1992 Nov 12
Externally publishedYes

Fingerprint

Vascular Smooth Muscle
Muscle
Actins
Muscle Cells
Modulation
Edetic Acid
Processing
Messenger RNA
Protein Isoforms
Cell Count
Muscles
Peptides
Biosynthesis
Labeling
Cysteine
Cell Differentiation
Skeletal Muscle
Phenotype

Keywords

  • actin
  • cell contacts
  • differentiation
  • EDTA
  • muscle cells
  • peptide mapping
  • posttranslational control

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Density-dependent modulation of vascular smooth muscle α-actin biosynthetic processing in differentiated BC3H1 myogenic cells. / Strauch, A. R.; Min, Bon Hong; Reeser, J. C.; Yan, H.; Foster, D. N.; Berman, M. D.

In: Journal of Cellular Biochemistry, Vol. 50, No. 3, 12.11.1992, p. 266-278.

Research output: Contribution to journalArticle

Strauch, A. R. ; Min, Bon Hong ; Reeser, J. C. ; Yan, H. ; Foster, D. N. ; Berman, M. D. / Density-dependent modulation of vascular smooth muscle α-actin biosynthetic processing in differentiated BC3H1 myogenic cells. In: Journal of Cellular Biochemistry. 1992 ; Vol. 50, No. 3. pp. 266-278.
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