Abstract
Macroalgae are considered to be promising biomass for fuels and chemicals production. To utilize brown macroalgae as biomass, the degradation of alginate, which is the main carbohydrate of brown macroalgae, into monomeric units is a critical prerequisite step. Saccharophagus degradans 2-40 is capable of degrading more than ten different polysaccharides including alginate, and its genome sequence demonstrated that this bacterium contains several putative alginate lyase genes including alg17C. The gene for Alg17C, which is classified into the PL-17 family, was cloned and overexpressed in Escherichia coli. The recombinant Alg17C was found to preferentially act on oligoalginates with degrees of polymerization higher than 2 to produce the alginate monomer, 4-deoxy-l-erythro-5-hexoseulose uronic acid. The optimal pH and temperature for Alg17C were found to be 6 and 40°C, respectively. The K M and V max of Alg17C were 35.2 mg/ml and 41.7 U/mg, respectively. Based on the results of this study, Alg17C could be used as the key enzyme to produce alginate monomers in the process of utilizing alginate for biofuels and chemicals production.
| Original language | English |
|---|---|
| Pages (from-to) | 2233-2239 |
| Number of pages | 7 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 93 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2012 Mar 1 |
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Keywords
- Alg17C
- Exo-type alginate lyase
- Oligoalginate lyase
- Polysaccharide lyase-17
- Saccharophagus degradans 2-40
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Depolymerization of alginate into a monomeric sugar acid using Alg17C, an exo-oligoalginate lyase cloned from Saccharophagus degradans 2-40. / Kim, Hee Taek; Chung, Jae Hyuk; Wang, Damao; Lee, Jieun; Woo, Hee Chul; Choi, In-Geol; Kim, Kyoung Heon.
In: Applied Microbiology and Biotechnology, Vol. 93, No. 5, 01.03.2012, p. 2233-2239.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Depolymerization of alginate into a monomeric sugar acid using Alg17C, an exo-oligoalginate lyase cloned from Saccharophagus degradans 2-40
AU - Kim, Hee Taek
AU - Chung, Jae Hyuk
AU - Wang, Damao
AU - Lee, Jieun
AU - Woo, Hee Chul
AU - Choi, In-Geol
AU - Kim, Kyoung Heon
PY - 2012/3/1
Y1 - 2012/3/1
N2 - Macroalgae are considered to be promising biomass for fuels and chemicals production. To utilize brown macroalgae as biomass, the degradation of alginate, which is the main carbohydrate of brown macroalgae, into monomeric units is a critical prerequisite step. Saccharophagus degradans 2-40 is capable of degrading more than ten different polysaccharides including alginate, and its genome sequence demonstrated that this bacterium contains several putative alginate lyase genes including alg17C. The gene for Alg17C, which is classified into the PL-17 family, was cloned and overexpressed in Escherichia coli. The recombinant Alg17C was found to preferentially act on oligoalginates with degrees of polymerization higher than 2 to produce the alginate monomer, 4-deoxy-l-erythro-5-hexoseulose uronic acid. The optimal pH and temperature for Alg17C were found to be 6 and 40°C, respectively. The K M and V max of Alg17C were 35.2 mg/ml and 41.7 U/mg, respectively. Based on the results of this study, Alg17C could be used as the key enzyme to produce alginate monomers in the process of utilizing alginate for biofuels and chemicals production.
AB - Macroalgae are considered to be promising biomass for fuels and chemicals production. To utilize brown macroalgae as biomass, the degradation of alginate, which is the main carbohydrate of brown macroalgae, into monomeric units is a critical prerequisite step. Saccharophagus degradans 2-40 is capable of degrading more than ten different polysaccharides including alginate, and its genome sequence demonstrated that this bacterium contains several putative alginate lyase genes including alg17C. The gene for Alg17C, which is classified into the PL-17 family, was cloned and overexpressed in Escherichia coli. The recombinant Alg17C was found to preferentially act on oligoalginates with degrees of polymerization higher than 2 to produce the alginate monomer, 4-deoxy-l-erythro-5-hexoseulose uronic acid. The optimal pH and temperature for Alg17C were found to be 6 and 40°C, respectively. The K M and V max of Alg17C were 35.2 mg/ml and 41.7 U/mg, respectively. Based on the results of this study, Alg17C could be used as the key enzyme to produce alginate monomers in the process of utilizing alginate for biofuels and chemicals production.
KW - Alg17C
KW - Exo-type alginate lyase
KW - Oligoalginate lyase
KW - Polysaccharide lyase-17
KW - Saccharophagus degradans 2-40
UR - http://www.scopus.com/inward/record.url?scp=84862789137&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862789137&partnerID=8YFLogxK
U2 - 10.1007/s00253-012-3882-x
DO - 10.1007/s00253-012-3882-x
M3 - Article
C2 - 22281843
AN - SCOPUS:84862789137
VL - 93
SP - 2233
EP - 2239
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 5
ER -