Depolymerization of alginate into a monomeric sugar acid using Alg17C, an exo-oligoalginate lyase cloned from Saccharophagus degradans 2-40

Hee Taek Kim, Jae Hyuk Chung, Damao Wang, Jieun Lee, Hee Chul Woo, In Geol Choi, Kyoung Heon Kim

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Macroalgae are considered to be promising biomass for fuels and chemicals production. To utilize brown macroalgae as biomass, the degradation of alginate, which is the main carbohydrate of brown macroalgae, into monomeric units is a critical prerequisite step. Saccharophagus degradans 2-40 is capable of degrading more than ten different polysaccharides including alginate, and its genome sequence demonstrated that this bacterium contains several putative alginate lyase genes including alg17C. The gene for Alg17C, which is classified into the PL-17 family, was cloned and overexpressed in Escherichia coli. The recombinant Alg17C was found to preferentially act on oligoalginates with degrees of polymerization higher than 2 to produce the alginate monomer, 4-deoxy-l-erythro-5-hexoseulose uronic acid. The optimal pH and temperature for Alg17C were found to be 6 and 40°C, respectively. The K M and V max of Alg17C were 35.2 mg/ml and 41.7 U/mg, respectively. Based on the results of this study, Alg17C could be used as the key enzyme to produce alginate monomers in the process of utilizing alginate for biofuels and chemicals production.

Original languageEnglish
Pages (from-to)2233-2239
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume93
Issue number5
DOIs
Publication statusPublished - 2012 Mar

Keywords

  • Alg17C
  • Exo-type alginate lyase
  • Oligoalginate lyase
  • Polysaccharide lyase-17
  • Saccharophagus degradans 2-40

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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