Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity

Mun Gyu Song, Hye Jin Lee, Bo Yeong Jin, Ruth Gutierrez-Aguilar, Kyung-Ho Shin, Sang-Hyun Choi, Sung Hee Um, Dong-Hun Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective Adipose tissue (AT) expansion requires AT remodeling, which depends on AT angiogenesis. Modulation of AT angiogenesis could have therapeutic promise for the treatment of obesity. However, it is unclear how the capacity of angiogenesis in each adipose depot is affected by over-nutrition. Therefore, we investigated the angiogenic capacity (AC) of subcutaneous and visceral fats in lean and obese mice. Methods We compared the AC of epididymal fat (EF) and inguinal fat (IF) using an angiogenesis assay in diet-induced obese (DIO) mice and diet-resistant (DR) mice fed a high-fat diet (HFD). Furthermore, we compared the expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation using RT-qPCR in the EF and IF of lean mice fed a low-fat diet (LFD), DIO mice, and DR mice fed a HFD. Results DIO mice showed a significant increase in the AC of EF only at 22 weeks of age compared to DR mice. The expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation were significantly higher in the EF of DIO mice than in those of LFD mice and DR mice, while expression levels of genes related to macrophages and their recruitment were higher in the IF of DIO mice than in those of LFD and DR mice. Expression of genes related to angiogenesis (including Hif1a, Vegfa, Fgf1, Kdr, and Pecam1), macrophage recruitment, and inflammation (including Emr1, Ccr2, Itgax, Ccl2, Tnf, and Il1b) correlated more strongly with body weight in the EF of HFD-fed obese mice compared to that of IF. Conclusions These results suggest depot-specific differences in AT angiogenesis and a potential role in the susceptibility to diet-induced obesity.

Original languageEnglish
Pages (from-to)1113-1120
Number of pages8
JournalMolecular Metabolism
Volume5
Issue number11
DOIs
Publication statusPublished - 2016 Nov 1

Fingerprint

Adipose Tissue
Obese Mice
Obesity
Diet
Fats
Groin
Fat-Restricted Diet
High Fat Diet
Macrophages
Gene Expression
Inflammation
Tissue Expansion
Intra-Abdominal Fat
Subcutaneous Fat
Body Weight
Therapeutics

Keywords

  • Adipose tissue
  • Angiogenesis
  • Diet-induced obese mice
  • Diet-resistant mice
  • High-fat diet
  • Inflammation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity. / Song, Mun Gyu; Lee, Hye Jin; Jin, Bo Yeong; Gutierrez-Aguilar, Ruth; Shin, Kyung-Ho; Choi, Sang-Hyun; Um, Sung Hee; Kim, Dong-Hun.

In: Molecular Metabolism, Vol. 5, No. 11, 01.11.2016, p. 1113-1120.

Research output: Contribution to journalArticle

Song, Mun Gyu ; Lee, Hye Jin ; Jin, Bo Yeong ; Gutierrez-Aguilar, Ruth ; Shin, Kyung-Ho ; Choi, Sang-Hyun ; Um, Sung Hee ; Kim, Dong-Hun. / Depot-specific differences in angiogenic capacity of adipose tissue in differential susceptibility to diet-induced obesity. In: Molecular Metabolism. 2016 ; Vol. 5, No. 11. pp. 1113-1120.
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AU - Shin, Kyung-Ho

AU - Choi, Sang-Hyun

AU - Um, Sung Hee

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AB - Objective Adipose tissue (AT) expansion requires AT remodeling, which depends on AT angiogenesis. Modulation of AT angiogenesis could have therapeutic promise for the treatment of obesity. However, it is unclear how the capacity of angiogenesis in each adipose depot is affected by over-nutrition. Therefore, we investigated the angiogenic capacity (AC) of subcutaneous and visceral fats in lean and obese mice. Methods We compared the AC of epididymal fat (EF) and inguinal fat (IF) using an angiogenesis assay in diet-induced obese (DIO) mice and diet-resistant (DR) mice fed a high-fat diet (HFD). Furthermore, we compared the expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation using RT-qPCR in the EF and IF of lean mice fed a low-fat diet (LFD), DIO mice, and DR mice fed a HFD. Results DIO mice showed a significant increase in the AC of EF only at 22 weeks of age compared to DR mice. The expression levels of genes related to angiogenesis, macrophage recruitment, and inflammation were significantly higher in the EF of DIO mice than in those of LFD mice and DR mice, while expression levels of genes related to macrophages and their recruitment were higher in the IF of DIO mice than in those of LFD and DR mice. Expression of genes related to angiogenesis (including Hif1a, Vegfa, Fgf1, Kdr, and Pecam1), macrophage recruitment, and inflammation (including Emr1, Ccr2, Itgax, Ccl2, Tnf, and Il1b) correlated more strongly with body weight in the EF of HFD-fed obese mice compared to that of IF. Conclusions These results suggest depot-specific differences in AT angiogenesis and a potential role in the susceptibility to diet-induced obesity.

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