Description of mycobacterium chelonae subsp. Bovis subsp. nov., isolated from cattle (bos taurus coreanae), emended description of mycobacterium chelonae and creation of mycobacterium chelonae subsp. chelonae subsp. nov

Byoung Jun Kim, Ga Na Kim, Bo Ram Kim, Che Ok Jeon, Joseph Jeong, Seon Ho Lee, Ji Hun Lim, Seung Heon Lee, Chang Ki Kim, Yoon Hoh Kook, Bum Joon Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Three rapidly growing mycobacterial strains, QIA-37T, QIA-40 and QIA-41, were isolated from the lymph nodes of three separate Korean native cattle, Hanwoo (Bos taurus coreanae). These strains were previously shown to be phylogenetically distinct but closely related to Mycobacterium chelonae ATCC 35752T by taxonomic approaches targeting three genes (16S rRNA, hsp6 and rpoB) and were further characterized using a polyphasic approach in this study. The 16S rRNA gene sequences of all three strains showed 99.7% sequence similarity with that of the M. chelonae type strain. A multilocus sequence typing analysis targeting 10 housekeeping genes, including hsp65 and rpoB, revealed a phylogenetic cluster of these strains with M. chelonae. DNA–DNA hybridization values of 78.2% between QIA-37T and M. chelonae indicated that it belongs to M. chelonae but is a novel subspecies distinct from M. chelonae. Phylogenetic analysis based on whole-genome sequences revealed a 95.44±0.06%average nucleotide identity (ANI) value with M. chelonae, slightly higher than the 95.0% ANI criterion for determining a novel species. In addition, distinct phenotypic characteristics such as positive growth at 37 °C, at which temperature M. chelonae does not grow, further support the taxonomic status of these strains as representatives of a novel subspecies of M. chelonae. Therefore, we propose an emended description of Mycobacterium chelonae, and descriptions of M. chelonae subsp. chelonae subsp. nov. and M. chelonae subsp. bovis subsp. nov. are presented; strains ATCC 35752T(=CCUG 47445T=CIP 104535T=DSM 43804T=JCM 6388T=NCTC 946T) and QIA-37T (=KCTC 39630T=JCM 30986T) are the type strains of the two novel subspecies.

Original languageEnglish
Article number002217
Pages (from-to)3882-3887
Number of pages6
JournalInternational journal of systematic and evolutionary microbiology
Volume67
Issue number10
DOIs
Publication statusPublished - 2017 Oct 1
Externally publishedYes

Fingerprint

aprepitant
Mycobacterium chelonae
new subspecies
subspecies
cattle
targeting
gene
phylogenetics
genome
Multilocus Sequence Typing
rRNA Genes
Nucleotides
temperature
nucleotides
ribosomal RNA

Keywords

  • Multilocus sequence typing
  • Mycobacterium chelonae
  • Mycobacterium chelonae subsp. Bovis
  • Mycobacterium chelonae subsp. chelonae
  • Rapid growing mycobacteria

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Description of mycobacterium chelonae subsp. Bovis subsp. nov., isolated from cattle (bos taurus coreanae), emended description of mycobacterium chelonae and creation of mycobacterium chelonae subsp. chelonae subsp. nov. / Kim, Byoung Jun; Kim, Ga Na; Kim, Bo Ram; Jeon, Che Ok; Jeong, Joseph; Lee, Seon Ho; Lim, Ji Hun; Lee, Seung Heon; Kim, Chang Ki; Kook, Yoon Hoh; Kim, Bum Joon.

In: International journal of systematic and evolutionary microbiology, Vol. 67, No. 10, 002217, 01.10.2017, p. 3882-3887.

Research output: Contribution to journalArticle

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title = "Description of mycobacterium chelonae subsp. Bovis subsp. nov., isolated from cattle (bos taurus coreanae), emended description of mycobacterium chelonae and creation of mycobacterium chelonae subsp. chelonae subsp. nov",
abstract = "Three rapidly growing mycobacterial strains, QIA-37T, QIA-40 and QIA-41, were isolated from the lymph nodes of three separate Korean native cattle, Hanwoo (Bos taurus coreanae). These strains were previously shown to be phylogenetically distinct but closely related to Mycobacterium chelonae ATCC 35752T by taxonomic approaches targeting three genes (16S rRNA, hsp6 and rpoB) and were further characterized using a polyphasic approach in this study. The 16S rRNA gene sequences of all three strains showed 99.7{\%} sequence similarity with that of the M. chelonae type strain. A multilocus sequence typing analysis targeting 10 housekeeping genes, including hsp65 and rpoB, revealed a phylogenetic cluster of these strains with M. chelonae. DNA–DNA hybridization values of 78.2{\%} between QIA-37T and M. chelonae indicated that it belongs to M. chelonae but is a novel subspecies distinct from M. chelonae. Phylogenetic analysis based on whole-genome sequences revealed a 95.44±0.06{\%}average nucleotide identity (ANI) value with M. chelonae, slightly higher than the 95.0{\%} ANI criterion for determining a novel species. In addition, distinct phenotypic characteristics such as positive growth at 37 °C, at which temperature M. chelonae does not grow, further support the taxonomic status of these strains as representatives of a novel subspecies of M. chelonae. Therefore, we propose an emended description of Mycobacterium chelonae, and descriptions of M. chelonae subsp. chelonae subsp. nov. and M. chelonae subsp. bovis subsp. nov. are presented; strains ATCC 35752T(=CCUG 47445T=CIP 104535T=DSM 43804T=JCM 6388T=NCTC 946T) and QIA-37T (=KCTC 39630T=JCM 30986T) are the type strains of the two novel subspecies.",
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AU - Kim, Byoung Jun

AU - Kim, Ga Na

AU - Kim, Bo Ram

AU - Jeon, Che Ok

AU - Jeong, Joseph

AU - Lee, Seon Ho

AU - Lim, Ji Hun

AU - Lee, Seung Heon

AU - Kim, Chang Ki

AU - Kook, Yoon Hoh

AU - Kim, Bum Joon

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N2 - Three rapidly growing mycobacterial strains, QIA-37T, QIA-40 and QIA-41, were isolated from the lymph nodes of three separate Korean native cattle, Hanwoo (Bos taurus coreanae). These strains were previously shown to be phylogenetically distinct but closely related to Mycobacterium chelonae ATCC 35752T by taxonomic approaches targeting three genes (16S rRNA, hsp6 and rpoB) and were further characterized using a polyphasic approach in this study. The 16S rRNA gene sequences of all three strains showed 99.7% sequence similarity with that of the M. chelonae type strain. A multilocus sequence typing analysis targeting 10 housekeeping genes, including hsp65 and rpoB, revealed a phylogenetic cluster of these strains with M. chelonae. DNA–DNA hybridization values of 78.2% between QIA-37T and M. chelonae indicated that it belongs to M. chelonae but is a novel subspecies distinct from M. chelonae. Phylogenetic analysis based on whole-genome sequences revealed a 95.44±0.06%average nucleotide identity (ANI) value with M. chelonae, slightly higher than the 95.0% ANI criterion for determining a novel species. In addition, distinct phenotypic characteristics such as positive growth at 37 °C, at which temperature M. chelonae does not grow, further support the taxonomic status of these strains as representatives of a novel subspecies of M. chelonae. Therefore, we propose an emended description of Mycobacterium chelonae, and descriptions of M. chelonae subsp. chelonae subsp. nov. and M. chelonae subsp. bovis subsp. nov. are presented; strains ATCC 35752T(=CCUG 47445T=CIP 104535T=DSM 43804T=JCM 6388T=NCTC 946T) and QIA-37T (=KCTC 39630T=JCM 30986T) are the type strains of the two novel subspecies.

AB - Three rapidly growing mycobacterial strains, QIA-37T, QIA-40 and QIA-41, were isolated from the lymph nodes of three separate Korean native cattle, Hanwoo (Bos taurus coreanae). These strains were previously shown to be phylogenetically distinct but closely related to Mycobacterium chelonae ATCC 35752T by taxonomic approaches targeting three genes (16S rRNA, hsp6 and rpoB) and were further characterized using a polyphasic approach in this study. The 16S rRNA gene sequences of all three strains showed 99.7% sequence similarity with that of the M. chelonae type strain. A multilocus sequence typing analysis targeting 10 housekeeping genes, including hsp65 and rpoB, revealed a phylogenetic cluster of these strains with M. chelonae. DNA–DNA hybridization values of 78.2% between QIA-37T and M. chelonae indicated that it belongs to M. chelonae but is a novel subspecies distinct from M. chelonae. Phylogenetic analysis based on whole-genome sequences revealed a 95.44±0.06%average nucleotide identity (ANI) value with M. chelonae, slightly higher than the 95.0% ANI criterion for determining a novel species. In addition, distinct phenotypic characteristics such as positive growth at 37 °C, at which temperature M. chelonae does not grow, further support the taxonomic status of these strains as representatives of a novel subspecies of M. chelonae. Therefore, we propose an emended description of Mycobacterium chelonae, and descriptions of M. chelonae subsp. chelonae subsp. nov. and M. chelonae subsp. bovis subsp. nov. are presented; strains ATCC 35752T(=CCUG 47445T=CIP 104535T=DSM 43804T=JCM 6388T=NCTC 946T) and QIA-37T (=KCTC 39630T=JCM 30986T) are the type strains of the two novel subspecies.

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