Design and characterization of a maltose binding protein-linked growth factor for matrix engineering

Min Han, In Su Park, Soo Hyun Kim, Byung Soo Kim, Sang Heon Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 1019 ng/cm2 at 100 μg protein/ml. The phosphorylation of VEGF receptors in the MBP-VEGF stimulated HEK293/KDR cells as depicted from western blot analysis. Cell adhesion to a MBP-VEGF immobilized surface was mediated by the VEGF-VEGFR interaction. These results suggest that MBP-VEGFs are active and a MBP immobilization system can then anchor various bioactive proteins to hydrophobic surfaces.

Original languageEnglish
Pages (from-to)1677-1684
Number of pages8
JournalBiotechnology letters
Volume31
Issue number11
DOIs
Publication statusPublished - 2009 Oct
Externally publishedYes

Keywords

  • Artificial matrix
  • Cell adhesion
  • Growth factor
  • Maltose-binding protein

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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