Design and investigation of nanoemulsified carrier based on amphiphile-modified hyaluronic acid

Ming Kong, Xiguang Chen, Hyun Jin Park

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Hyaluronic acid (HA) was modified into an amphiphile through esterification between the carboxyl and hydroxyl moieties on glycerol-α-monostearate (GMS). The conjugation was verified via FT/IR and 1H NMR. Ultrasonification and solvent evaporation were used to prepare a fine oil/water/surfactant (O/W/S) nanoemulsion consisting of methylene chloride as the oil phase (O), HA water solution (W), and non-ionic Tween-80 and Span-20 as surfactant (S). The optimal ratio of the disperse phase was screened in nanoemulsion formed without HA-GMS. Tween-80 and Span-20 at the required hydrophilic-lipophilic balance (HLB) of 12.5 and a ratio of O/S (ROS) of 60, which accounted for 5% (w/w) in the emulsion system, was determined to be the optimal disperse phase ratio, giving rise to the smallest droplet size (39.7 nm) and lower protein dispersibility (0.341). The smallest droplet size of the HA-GMS nanoemulsion was 42.0 nm, with contraction to 38.9 nm evident after 96 h storage. Higher degree of substitution or lower molecular weight was favorable for the formation of smaller emulsified droplets. Nanoemulsions were characterized morphologically by electronic microscopy.

Original languageEnglish
Pages (from-to)462-469
Number of pages8
JournalCarbohydrate Polymers
Volume83
Issue number2
DOIs
Publication statusPublished - 2011 Jan 10

Fingerprint

Hyaluronic acid
Amphiphiles
Hyaluronic Acid
Glycerol
Polysorbates
Surface-Active Agents
Oils
Surface active agents
Water
Methylene Chloride
Dichloromethane
Esterification
Emulsions
Hydroxyl Radical
Microscopic examination
Evaporation
Substitution reactions
Molecular weight
Nuclear magnetic resonance
Proteins

Keywords

  • Hyaluronic acid
  • Methylene chloride
  • Monostearin
  • Nanoemulsion
  • Solvent evaporation

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

Design and investigation of nanoemulsified carrier based on amphiphile-modified hyaluronic acid. / Kong, Ming; Chen, Xiguang; Park, Hyun Jin.

In: Carbohydrate Polymers, Vol. 83, No. 2, 10.01.2011, p. 462-469.

Research output: Contribution to journalArticle

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