TY - CHAP
T1 - Design and synthesis of biofunctionalized metallic/magnetic nanomaterials
AU - Lim, Eun Kyung
AU - Haam, Seungjoo
AU - Lee, Kwangyeol
AU - Huh, Yong Min
PY - 2011
Y1 - 2011
N2 - Organic solvent-soluble nanocrystals suitable for magnetic resonance imaging are prepared by two routes, namely, a coprecipitation method and a multiple-step thermal decomposition method (seed-mediated growth). The size, shape, crystallinity, phase, and composition of the prepared nanocrystals are determined by various characterization techniques, including transmission electron microscopy, vibratingsample magnetometer, X-ray diffraction, and inductively coupled plasma mass spectroscopy. Subsequently, the organic-soluble nanocrystals are rendered water-soluble by two methods, the microemulsion method and the ligand exchange method, for biomedical applications. Detailed protocols for the preparation of water-soluble nanocrystals, as well as procedures for drug-loading and antibody conjugation to the water-soluble nanocrystals are provided.
AB - Organic solvent-soluble nanocrystals suitable for magnetic resonance imaging are prepared by two routes, namely, a coprecipitation method and a multiple-step thermal decomposition method (seed-mediated growth). The size, shape, crystallinity, phase, and composition of the prepared nanocrystals are determined by various characterization techniques, including transmission electron microscopy, vibratingsample magnetometer, X-ray diffraction, and inductively coupled plasma mass spectroscopy. Subsequently, the organic-soluble nanocrystals are rendered water-soluble by two methods, the microemulsion method and the ligand exchange method, for biomedical applications. Detailed protocols for the preparation of water-soluble nanocrystals, as well as procedures for drug-loading and antibody conjugation to the water-soluble nanocrystals are provided.
KW - Antibody
KW - Coprecipitation
KW - Drug
KW - Ligand exchange
KW - Magnetic nanocrystals
KW - Magnetic nanoparticles
KW - Microemulsion
KW - Seed-mediated growth
UR - http://www.scopus.com/inward/record.url?scp=80053296434&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053296434&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-151-2_36
DO - 10.1007/978-1-61779-151-2_36
M3 - Chapter
C2 - 21674356
AN - SCOPUS:80053296434
T3 - Methods in Molecular Biology
SP - 583
EP - 595
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -