Designed hybridization properties of DNA-gold nanoparticle conjugates for the ultraselective detection of a single-base mutation in the breast cancer gene BRCA1.

Ju Hwan Oh, Jae-Seung Lee

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

We have investigated the hybridization properties of DNA-gold nanoparticle conjugates and have discovered that the hybridization properties are dramatically affected by controlling various synthetic and environmental conditions. We have further demonstrated that moderate DNA loading instead of high loading per nanoparticle significantly enhances the hybridization rates of DNA-gold nanoparticle conjugates, which allows one to precisely design their hybridization properties to distinguish a single-nucleotide polymorphism (SNP). A diagnostic application for the colorimetric detection of an SNP associated with a mutation in the breast cancer gene BRCA1 has been carefully designed and demonstrated.

Original languageEnglish
Pages (from-to)7364-7370
Number of pages7
JournalAnalytical Chemistry
Volume83
Issue number19
Publication statusPublished - 2011 Oct 1

Fingerprint

Gold
Genes
Nanoparticles
Polymorphism
DNA
Nucleotides

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

@article{bb2d5abe6a76424faf2fc35f7c4bd89b,
title = "Designed hybridization properties of DNA-gold nanoparticle conjugates for the ultraselective detection of a single-base mutation in the breast cancer gene BRCA1.",
abstract = "We have investigated the hybridization properties of DNA-gold nanoparticle conjugates and have discovered that the hybridization properties are dramatically affected by controlling various synthetic and environmental conditions. We have further demonstrated that moderate DNA loading instead of high loading per nanoparticle significantly enhances the hybridization rates of DNA-gold nanoparticle conjugates, which allows one to precisely design their hybridization properties to distinguish a single-nucleotide polymorphism (SNP). A diagnostic application for the colorimetric detection of an SNP associated with a mutation in the breast cancer gene BRCA1 has been carefully designed and demonstrated.",
author = "Oh, {Ju Hwan} and Jae-Seung Lee",
year = "2011",
month = "10",
day = "1",
language = "English",
volume = "83",
pages = "7364--7370",
journal = "The BMJ",
issn = "0730-6512",
publisher = "Kluwer Academic Publishers",
number = "19",

}

TY - JOUR

T1 - Designed hybridization properties of DNA-gold nanoparticle conjugates for the ultraselective detection of a single-base mutation in the breast cancer gene BRCA1.

AU - Oh, Ju Hwan

AU - Lee, Jae-Seung

PY - 2011/10/1

Y1 - 2011/10/1

N2 - We have investigated the hybridization properties of DNA-gold nanoparticle conjugates and have discovered that the hybridization properties are dramatically affected by controlling various synthetic and environmental conditions. We have further demonstrated that moderate DNA loading instead of high loading per nanoparticle significantly enhances the hybridization rates of DNA-gold nanoparticle conjugates, which allows one to precisely design their hybridization properties to distinguish a single-nucleotide polymorphism (SNP). A diagnostic application for the colorimetric detection of an SNP associated with a mutation in the breast cancer gene BRCA1 has been carefully designed and demonstrated.

AB - We have investigated the hybridization properties of DNA-gold nanoparticle conjugates and have discovered that the hybridization properties are dramatically affected by controlling various synthetic and environmental conditions. We have further demonstrated that moderate DNA loading instead of high loading per nanoparticle significantly enhances the hybridization rates of DNA-gold nanoparticle conjugates, which allows one to precisely design their hybridization properties to distinguish a single-nucleotide polymorphism (SNP). A diagnostic application for the colorimetric detection of an SNP associated with a mutation in the breast cancer gene BRCA1 has been carefully designed and demonstrated.

UR - http://www.scopus.com/inward/record.url?scp=84862833396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862833396&partnerID=8YFLogxK

M3 - Article

VL - 83

SP - 7364

EP - 7370

JO - The BMJ

JF - The BMJ

SN - 0730-6512

IS - 19

ER -