Designing dynamic surfaces for regulation of biological responses

Ji-Hun Seo, Sachiro Kakinoki, Yuuki Inoue, Tetsuji Yamaoka, Kazuhiko Ishihara, Nobuhiko Yui

Research output: Contribution to journalReview article

30 Citations (Scopus)

Abstract

ABA block copolymers composed of highly methylated polyrotaxane and hydrophobic anchoring terminal segments containing 2-methacryloyloxyethyl phosphorylcholine (MPC) and n-butyl methacrylate (PMB) (OMe-PRX-PMB) were synthesized as a platform of molecularly dynamic biomaterials. A contact angle measurement indicated that polymer surfaces with higher molecular mobility factors (Mf) estimated from quartz crystal microbalance with dissipation (QCM-D) measurements showed more significant changes in hydrophilicity in response to an environmental change between air and water; the OMe-PRX-PMB surface showed the highest Mf among the prepared polymer surfaces. Fibrinogen adsorption and its conformational analysis estimated by QCM-D and enzyme-linked immunosorbent assay revealed that large amounts of fibrinogen adsorption occurred in a soft manner on the OMe-PRX-PMB surface and that the antibody binding to the C-terminus of the fibrinogen γ chains responsible for platelet adhesion and activation decreased as the Mf value increased. Furthermore, it was found that the OMe-PRX-PMB surface showed low platelet adhesion and high fibroblast adhesion, suggesting that molecular movement on biomaterial surfaces could be one of the key parameters in the regulation of a non-specific biological response.

Original languageEnglish
Pages (from-to)5477-5485
Number of pages9
JournalSoft Matter
Volume8
Issue number20
DOIs
Publication statusPublished - 2012 May 28
Externally publishedYes

Fingerprint

fibrinogen
Fibrinogen
adhesion
Adhesion
Quartz crystal microbalances
Biocompatible Materials
Platelets
platelets
quartz crystals
microbalances
Polymers
dissipation
Adsorption
Immunosorbents
adsorption
Hydrophilicity
fibroblasts
polymers
Fibroblasts
Angle measurement

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Seo, J-H., Kakinoki, S., Inoue, Y., Yamaoka, T., Ishihara, K., & Yui, N. (2012). Designing dynamic surfaces for regulation of biological responses. Soft Matter, 8(20), 5477-5485. https://doi.org/10.1039/c2sm25318f

Designing dynamic surfaces for regulation of biological responses. / Seo, Ji-Hun; Kakinoki, Sachiro; Inoue, Yuuki; Yamaoka, Tetsuji; Ishihara, Kazuhiko; Yui, Nobuhiko.

In: Soft Matter, Vol. 8, No. 20, 28.05.2012, p. 5477-5485.

Research output: Contribution to journalReview article

Seo, J-H, Kakinoki, S, Inoue, Y, Yamaoka, T, Ishihara, K & Yui, N 2012, 'Designing dynamic surfaces for regulation of biological responses', Soft Matter, vol. 8, no. 20, pp. 5477-5485. https://doi.org/10.1039/c2sm25318f
Seo J-H, Kakinoki S, Inoue Y, Yamaoka T, Ishihara K, Yui N. Designing dynamic surfaces for regulation of biological responses. Soft Matter. 2012 May 28;8(20):5477-5485. https://doi.org/10.1039/c2sm25318f
Seo, Ji-Hun ; Kakinoki, Sachiro ; Inoue, Yuuki ; Yamaoka, Tetsuji ; Ishihara, Kazuhiko ; Yui, Nobuhiko. / Designing dynamic surfaces for regulation of biological responses. In: Soft Matter. 2012 ; Vol. 8, No. 20. pp. 5477-5485.
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