Designing dynamic surfaces for regulation of biological responses

Ji Hun Seo, Sachiro Kakinoki, Yuuki Inoue, Tetsuji Yamaoka, Kazuhiko Ishihara, Nobuhiko Yui

Research output: Contribution to journalReview article

33 Citations (Scopus)

Abstract

ABA block copolymers composed of highly methylated polyrotaxane and hydrophobic anchoring terminal segments containing 2-methacryloyloxyethyl phosphorylcholine (MPC) and n-butyl methacrylate (PMB) (OMe-PRX-PMB) were synthesized as a platform of molecularly dynamic biomaterials. A contact angle measurement indicated that polymer surfaces with higher molecular mobility factors (Mf) estimated from quartz crystal microbalance with dissipation (QCM-D) measurements showed more significant changes in hydrophilicity in response to an environmental change between air and water; the OMe-PRX-PMB surface showed the highest Mf among the prepared polymer surfaces. Fibrinogen adsorption and its conformational analysis estimated by QCM-D and enzyme-linked immunosorbent assay revealed that large amounts of fibrinogen adsorption occurred in a soft manner on the OMe-PRX-PMB surface and that the antibody binding to the C-terminus of the fibrinogen γ chains responsible for platelet adhesion and activation decreased as the Mf value increased. Furthermore, it was found that the OMe-PRX-PMB surface showed low platelet adhesion and high fibroblast adhesion, suggesting that molecular movement on biomaterial surfaces could be one of the key parameters in the regulation of a non-specific biological response.

Original languageEnglish
Pages (from-to)5477-5485
Number of pages9
JournalSoft Matter
Volume8
Issue number20
DOIs
Publication statusPublished - 2012 May 28

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

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    Seo, J. H., Kakinoki, S., Inoue, Y., Yamaoka, T., Ishihara, K., & Yui, N. (2012). Designing dynamic surfaces for regulation of biological responses. Soft Matter, 8(20), 5477-5485. https://doi.org/10.1039/c2sm25318f