Desipramine and citalopram attenuate pretest swim-induced increases in prodynorphin immunoreactivity in the dorsal bed nucleus of the stria terminalis and the lateral division of the central nucleus of the amygdala in the forced swimming test

Sung Chung, Hee Jeong Kim, Hyun Ju Kim, Sun Hye Choi, Jin Hee Cho, Yun Ha Cho, Dong-Hun Kim, Kyung-Ho Shin

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Dynorphin in the nucleus accumbens shell plays an important role in antidepressant-like effect in the forced swimming test (FST), but it is unclear whether desipramine and citalopram treatments alter prodynorphin levels in other brain areas. To explore this possibility, we injected mice with desipramine and citalopram 0.5, 19, and 23h after a 15-min pretest swim and observed changes in prodynorphin expression before the test swim, which was conducted 24h after the pretest swim. The pretest swim increased prodynorphin immunoreactivity in the dorsal bed nucleus of the stria terminalis (dBNST) and lateral division of the central nucleus of the amygdala (CeL). This increase in prodynorphin immunoreactivity in the dBNST and CeL was blocked by desipramine and citalopram treatments. Similar changes in prodynorphin mRNA levels were observed in the dBNST and CeL, but these changes did not reach significance. To understand the underlying mechanism, we assessed changes in phosphorylated CREB at Ser133 (pCREB) immunoreactivity in the dBNST and central nucleus of the amygdala (CeA). Treatment with citalopram but not desipramine after the pretest swim significantly increased pCREB immunoreactivity only in the dBNST. These results suggest that regulation of prodynorphin in the dBNST and CeL before the test swim may be involved in the antidepressant-like effect of desipramine and citalopram in the FST and suggest that changes in pCREB immunoreactivity in these areas may not play an important role in the regulation of prodynorphin in the dBNST and CeA.

Original languageEnglish
Pages (from-to)273-280
Number of pages8
JournalNeuropeptides
Volume48
Issue number5
DOIs
Publication statusPublished - 2014 Oct 1

Keywords

  • Amygdala
  • Antidepressive agents
  • Bed nucleus of stria terminalis
  • FST
  • Prodynorphin

ASJC Scopus subject areas

  • Endocrinology
  • Neurology
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Medicine(all)

Fingerprint Dive into the research topics of 'Desipramine and citalopram attenuate pretest swim-induced increases in prodynorphin immunoreactivity in the dorsal bed nucleus of the stria terminalis and the lateral division of the central nucleus of the amygdala in the forced swimming test'. Together they form a unique fingerprint.

Cite this