Desipramine inhibits histamine H1 receptor-induced Ca2+ signaling in rat hypothalamic cells

Ji Ah Kang, Keimin Lee, Kwang Min Lee, Sukhee Cho, Jinsoo Seo, Eun Mi Hur, Chul Seung Park, Ja-Hyun Baik, Se Young Choi

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4 Citations (Scopus)

Abstract

The hypothalamus in the brain is the main center for appetite control and integrates signals from adipose tissue and the gastrointestinal tract. Antidepressants are known to modulate the activities of hypothalamic neurons and affect food intake, but the cellular and molecular mechanisms by which antidepressants modulate hypothalamic function remain unclear. Here we have investigated how hypothalamic neurons respond to treatment with antidepressants, including desipramine and sibutramine. In primary cultured rat hypothalamic cells, desipramine markedly suppressed the elevation of intracellular Ca2+ evoked by histamine H1 receptor activation. Desipramine also inhibited the histamine-induced Ca2+ increase and the expression of corticotrophin-releasing hormone in hypothalamic GT1-1 cells. The effect of desipramine was not affected by pretreatment with prazosin or propranolol, excluding catecholamine reuptake activity of desipramine as an underlying mechanism. Sibutramine which is also an antidepressant but decreases food intake, had little effect on the histamine-induced Ca2+ increase or AMP-activated protein kinase activity. Our results reveal that desipramine and sibutramine have different effects on histamine H1 receptor signaling in hypothalamic cells and suggest that distinct regulation of hypothalamic histamine signaling might underlie the differential regulation of food intake between antidepressants.

Original languageEnglish
Article numbere36185
JournalPLoS One
Volume7
Issue number4
DOIs
Publication statusPublished - 2012 Apr 26

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Kang, J. A., Lee, K., Lee, K. M., Cho, S., Seo, J., Hur, E. M., Park, C. S., Baik, J-H., & Choi, S. Y. (2012). Desipramine inhibits histamine H1 receptor-induced Ca2+ signaling in rat hypothalamic cells. PLoS One, 7(4), [e36185]. https://doi.org/10.1371/journal.pone.0036185