Abstract
The rhamnolipid biosurfactant-producing bacterium, strain SDRB-G7, was isolated from the sediment of Sindu-ri beach and identified as Rhodococcus fascians based on a phylogenetic analysis. Optimal activity, with the highest yield (2.441 g/L) and surface tension-reducing activity (24.38 mN/m), was observed when the cells were grown on olive oil as their sole source of carbon at pH 8.0. The rhamnolipid biosurfactant showed environmental stability at a variety of NaCl concentrations (2-20%) and pH values (2-12) even under acidic conditions. Of the initial anthracene, 66% was solubilized by 100% crude biosurfactant. Furthermore, 100% crude biosurfactant desorbed 81% of the anthracene in sediment into the aqueous phase. These results suggest that the rhamnolipid biosurfactant produced from R. fascians SDRB-G7 is a promising candidate for polycyclic aromatic hydrocarbon (PAH) removal from the sediment and can be an effective agent for processes that bioremediate PAHs such as surfactant-enhanced remediation. Practitioner points: Biosurfactants can accelerate desorption of PAHs and improve their solubility. BS-producing R. fascians SDRB-G7 was selected by screening of biochemical tests. Solubility of anthracene was enhanced by rhamnolipid produced by strain SDRB-G7. Microbial surfactant is a promising alternative for bioremediation of PAH-polluted sites.
| Original language | English |
|---|---|
| Journal | Water Environment Research |
| DOIs | |
| Publication status | Published - 2019 Jan 1 |
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Keywords
- biosurfactant-producing bacteria
- desorption
- marine sediment
- PAHs
- rhamnolipid
- solubilization
ASJC Scopus subject areas
- Environmental Chemistry
- Ecological Modelling
- Water Science and Technology
- Waste Management and Disposal
- Pollution
Cite this
Desorption and solubilization of anthracene by a rhamnolipid biosurfactant from Rhodococcus fascians. / Kim, Chul Hwan; Lee, Dong Wan; Heo, Young Mok; Lee, Hanbyul; Yoo, Yeonjae; Kim, Gyu Hyeok; Kim, Jae-Jin.
In: Water Environment Research, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Desorption and solubilization of anthracene by a rhamnolipid biosurfactant from Rhodococcus fascians
AU - Kim, Chul Hwan
AU - Lee, Dong Wan
AU - Heo, Young Mok
AU - Lee, Hanbyul
AU - Yoo, Yeonjae
AU - Kim, Gyu Hyeok
AU - Kim, Jae-Jin
PY - 2019/1/1
Y1 - 2019/1/1
N2 - The rhamnolipid biosurfactant-producing bacterium, strain SDRB-G7, was isolated from the sediment of Sindu-ri beach and identified as Rhodococcus fascians based on a phylogenetic analysis. Optimal activity, with the highest yield (2.441 g/L) and surface tension-reducing activity (24.38 mN/m), was observed when the cells were grown on olive oil as their sole source of carbon at pH 8.0. The rhamnolipid biosurfactant showed environmental stability at a variety of NaCl concentrations (2-20%) and pH values (2-12) even under acidic conditions. Of the initial anthracene, 66% was solubilized by 100% crude biosurfactant. Furthermore, 100% crude biosurfactant desorbed 81% of the anthracene in sediment into the aqueous phase. These results suggest that the rhamnolipid biosurfactant produced from R. fascians SDRB-G7 is a promising candidate for polycyclic aromatic hydrocarbon (PAH) removal from the sediment and can be an effective agent for processes that bioremediate PAHs such as surfactant-enhanced remediation. Practitioner points: Biosurfactants can accelerate desorption of PAHs and improve their solubility. BS-producing R. fascians SDRB-G7 was selected by screening of biochemical tests. Solubility of anthracene was enhanced by rhamnolipid produced by strain SDRB-G7. Microbial surfactant is a promising alternative for bioremediation of PAH-polluted sites.
AB - The rhamnolipid biosurfactant-producing bacterium, strain SDRB-G7, was isolated from the sediment of Sindu-ri beach and identified as Rhodococcus fascians based on a phylogenetic analysis. Optimal activity, with the highest yield (2.441 g/L) and surface tension-reducing activity (24.38 mN/m), was observed when the cells were grown on olive oil as their sole source of carbon at pH 8.0. The rhamnolipid biosurfactant showed environmental stability at a variety of NaCl concentrations (2-20%) and pH values (2-12) even under acidic conditions. Of the initial anthracene, 66% was solubilized by 100% crude biosurfactant. Furthermore, 100% crude biosurfactant desorbed 81% of the anthracene in sediment into the aqueous phase. These results suggest that the rhamnolipid biosurfactant produced from R. fascians SDRB-G7 is a promising candidate for polycyclic aromatic hydrocarbon (PAH) removal from the sediment and can be an effective agent for processes that bioremediate PAHs such as surfactant-enhanced remediation. Practitioner points: Biosurfactants can accelerate desorption of PAHs and improve their solubility. BS-producing R. fascians SDRB-G7 was selected by screening of biochemical tests. Solubility of anthracene was enhanced by rhamnolipid produced by strain SDRB-G7. Microbial surfactant is a promising alternative for bioremediation of PAH-polluted sites.
KW - biosurfactant-producing bacteria
KW - desorption
KW - marine sediment
KW - PAHs
KW - rhamnolipid
KW - solubilization
UR - http://www.scopus.com/inward/record.url?scp=85064718126&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064718126&partnerID=8YFLogxK
U2 - 10.1002/wer.1103
DO - 10.1002/wer.1103
M3 - Article
C2 - 30874337
AN - SCOPUS:85064718126
JO - Water environment research : a research publication of the Water Environment Federation
JF - Water environment research : a research publication of the Water Environment Federation
SN - 1061-4303
ER -