Detecting oxidized contaminants in water using sulfur-oxidizing bacteria

Steven W. Van Ginkel, Sedky H A Hassan, Yong Sik Ok, Jae E. Yang, Yong Seong Kim, Sang Eun Oh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

For the rapid and reliable detection of oxidized contaminants (i.e., nitrite, nitrate, perchlorate, dichromate) in water, a novel toxicity detection methodology based on sulfur-oxidizing bacteria (SOB) has been developed. The methodology exploits the ability of SOB to oxidize elemental sulfur to sulfuric acid in the presence of oxygen. The reaction results in an increase in electrical conductivity (EC) and a decrease in pH. When oxidized contaminants were added to the system, the effluent EC decreased and the pH increased due to the inhibition of the SOB. We found that the system can detect these contaminants in the 5-50 ppb range (in the case of NO3 -, 10 ppm was detected), which is lower than many whole-cell biosensors to date. At low pH, the oxidized contaminants are mostly in their acid or nonpolar, protonated form which act as uncouplers and make the SOB biosensor more sensitive than other whole-cell biosensors which operate at higher pH values where the contaminants exist as dissociated anions. The SOB biosensor can detect toxicity on the order of minutes to hours which can serve as an early warning so as to not pollute the environment and affect public health.

Original languageEnglish
Pages (from-to)3739-3745
Number of pages7
JournalEnvironmental Science and Technology
Volume45
Issue number8
DOIs
Publication statusPublished - 2011 Apr 15
Externally publishedYes

Fingerprint

Sulfur
Bacteria
sulfur
Biosensing Techniques
Impurities
Biosensors
bacterium
pollutant
Water
Electric Conductivity
water
electrical conductivity
Toxicity
Environment and Public Health
toxicity
perchlorate
methodology
Public health
Nitrites
Nitrates

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Van Ginkel, S. W., Hassan, S. H. A., Ok, Y. S., Yang, J. E., Kim, Y. S., & Oh, S. E. (2011). Detecting oxidized contaminants in water using sulfur-oxidizing bacteria. Environmental Science and Technology, 45(8), 3739-3745. https://doi.org/10.1021/es1036892

Detecting oxidized contaminants in water using sulfur-oxidizing bacteria. / Van Ginkel, Steven W.; Hassan, Sedky H A; Ok, Yong Sik; Yang, Jae E.; Kim, Yong Seong; Oh, Sang Eun.

In: Environmental Science and Technology, Vol. 45, No. 8, 15.04.2011, p. 3739-3745.

Research output: Contribution to journalArticle

Van Ginkel, Steven W. ; Hassan, Sedky H A ; Ok, Yong Sik ; Yang, Jae E. ; Kim, Yong Seong ; Oh, Sang Eun. / Detecting oxidized contaminants in water using sulfur-oxidizing bacteria. In: Environmental Science and Technology. 2011 ; Vol. 45, No. 8. pp. 3739-3745.
@article{6a22995ae4074ea5a0d27d8dc9b48293,
title = "Detecting oxidized contaminants in water using sulfur-oxidizing bacteria",
abstract = "For the rapid and reliable detection of oxidized contaminants (i.e., nitrite, nitrate, perchlorate, dichromate) in water, a novel toxicity detection methodology based on sulfur-oxidizing bacteria (SOB) has been developed. The methodology exploits the ability of SOB to oxidize elemental sulfur to sulfuric acid in the presence of oxygen. The reaction results in an increase in electrical conductivity (EC) and a decrease in pH. When oxidized contaminants were added to the system, the effluent EC decreased and the pH increased due to the inhibition of the SOB. We found that the system can detect these contaminants in the 5-50 ppb range (in the case of NO3 -, 10 ppm was detected), which is lower than many whole-cell biosensors to date. At low pH, the oxidized contaminants are mostly in their acid or nonpolar, protonated form which act as uncouplers and make the SOB biosensor more sensitive than other whole-cell biosensors which operate at higher pH values where the contaminants exist as dissociated anions. The SOB biosensor can detect toxicity on the order of minutes to hours which can serve as an early warning so as to not pollute the environment and affect public health.",
author = "{Van Ginkel}, {Steven W.} and Hassan, {Sedky H A} and Ok, {Yong Sik} and Yang, {Jae E.} and Kim, {Yong Seong} and Oh, {Sang Eun}",
year = "2011",
month = "4",
day = "15",
doi = "10.1021/es1036892",
language = "English",
volume = "45",
pages = "3739--3745",
journal = "Environmental Science and Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "8",

}

TY - JOUR

T1 - Detecting oxidized contaminants in water using sulfur-oxidizing bacteria

AU - Van Ginkel, Steven W.

AU - Hassan, Sedky H A

AU - Ok, Yong Sik

AU - Yang, Jae E.

AU - Kim, Yong Seong

AU - Oh, Sang Eun

PY - 2011/4/15

Y1 - 2011/4/15

N2 - For the rapid and reliable detection of oxidized contaminants (i.e., nitrite, nitrate, perchlorate, dichromate) in water, a novel toxicity detection methodology based on sulfur-oxidizing bacteria (SOB) has been developed. The methodology exploits the ability of SOB to oxidize elemental sulfur to sulfuric acid in the presence of oxygen. The reaction results in an increase in electrical conductivity (EC) and a decrease in pH. When oxidized contaminants were added to the system, the effluent EC decreased and the pH increased due to the inhibition of the SOB. We found that the system can detect these contaminants in the 5-50 ppb range (in the case of NO3 -, 10 ppm was detected), which is lower than many whole-cell biosensors to date. At low pH, the oxidized contaminants are mostly in their acid or nonpolar, protonated form which act as uncouplers and make the SOB biosensor more sensitive than other whole-cell biosensors which operate at higher pH values where the contaminants exist as dissociated anions. The SOB biosensor can detect toxicity on the order of minutes to hours which can serve as an early warning so as to not pollute the environment and affect public health.

AB - For the rapid and reliable detection of oxidized contaminants (i.e., nitrite, nitrate, perchlorate, dichromate) in water, a novel toxicity detection methodology based on sulfur-oxidizing bacteria (SOB) has been developed. The methodology exploits the ability of SOB to oxidize elemental sulfur to sulfuric acid in the presence of oxygen. The reaction results in an increase in electrical conductivity (EC) and a decrease in pH. When oxidized contaminants were added to the system, the effluent EC decreased and the pH increased due to the inhibition of the SOB. We found that the system can detect these contaminants in the 5-50 ppb range (in the case of NO3 -, 10 ppm was detected), which is lower than many whole-cell biosensors to date. At low pH, the oxidized contaminants are mostly in their acid or nonpolar, protonated form which act as uncouplers and make the SOB biosensor more sensitive than other whole-cell biosensors which operate at higher pH values where the contaminants exist as dissociated anions. The SOB biosensor can detect toxicity on the order of minutes to hours which can serve as an early warning so as to not pollute the environment and affect public health.

UR - http://www.scopus.com/inward/record.url?scp=79954455536&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79954455536&partnerID=8YFLogxK

U2 - 10.1021/es1036892

DO - 10.1021/es1036892

M3 - Article

C2 - 21417357

AN - SCOPUS:79954455536

VL - 45

SP - 3739

EP - 3745

JO - Environmental Science and Technology

JF - Environmental Science and Technology

SN - 0013-936X

IS - 8

ER -