Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry

Yoontak Han, Eun Jin Lee

Research output: Contribution to journalArticlepeer-review

Abstract

The bacterial flagellum is an appendage structure that provides a means for motility to promote survival in fluctuating environments. For the intracellular pathogen Salmonella enterica serovar Typhimurium to survive within macrophages, flagellar gene expression must be tightly regulated, and thus, is controlled at multiple levels, including DNA recombination, transcription, post-transcription, protein synthesis, and assembly within host cells. To understand the contribution of flagella to Salmonella pathogenesis within the host, it is critical to detect flagella production within macrophages via microscopy. In this paper, we describe two methods for detecting bacterial flagella by microscopy both in vitro and in vivo infection models.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalJournal of Microbiology
Volume58
Issue number4
DOIs
Publication statusPublished - 2020 Apr 1

Keywords

  • Salmonella Typhimurium
  • Type II flagella

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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