Detection and assessment of clarithromycin inducible resistant strains among Korean mycobacterium abscessus clinical strains: PCR methods

Seung Heon Lee, Hee Kyung Yoo, Seol Hee Kim, Won Jung Koh, Chang Ki Kim, Young Kil Park, Hee Jin Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Mycobacterium abscessus group belongs to a group of rapidly growing mycobacteria (RGM) and, following Mycobacterium avium complex, is the second most common pathogen responsible for lung disease caused by nontuberculous mycobacteria (NTM). Clarithromycin is known to be the key drug in the treatment of M. abscessus group disease, but a high failure rate of treatment response is reported due to clarithromycin inducible resistance. Methods: Using the results from a clarithromycin susceptibility test we examined the proportion of clarithromycin inducible resistant M. abscessus (sensu stricto; hereafter referred to as M. abscessus) clinical strains. Also, we attempted to detect the clarithromycin resistant strains, using the amplification refractory mutation system-PCR (ARMS-PCR) and real-time PCR methods for rapid detection of single-nucleotide polymorphisms (SNPs) at position 28 (T or C) of the erm(41) gene of M. abscessus leading to resistance to clarithromycin. Results: Of the 157 M. abscessus clinical strains, clarithromycin susceptible, resistant, and inducible resistant strains accounted for 10.83% (n = 17), 22.29% (n = 35), and 66.88% (n = 105), respectively. Clarithromycin resistant strains were able to separate from clarithromycin susceptible strains by ARMS-PCR and real-time PCR identical to DNA sequence analysis. Conclusion: Most M. abscessus clinical strains in Korea are resistant to clarithromycin, and ARMS-PCR and real-time PCR are useful tools for the rapid detection of single-nucleotide polymorphisms (SNPs) at position 28 of the erm(41) gene.

Original languageEnglish
Pages (from-to)409-414
Number of pages6
JournalJournal of Clinical Laboratory Analysis
Volume28
Issue number5
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Fingerprint

Clarithromycin
Mycobacterium
Polymerase Chain Reaction
Refractory materials
Amplification
Real-Time Polymerase Chain Reaction
Polymorphism
Mutation
Single Nucleotide Polymorphism
Nucleotides
Genes
Mycobacterium avium Complex
Nontuberculous Mycobacteria
Pulmonary diseases
DNA sequences
Pathogens
Korea
Treatment Failure
DNA Sequence Analysis
Lung Diseases

Keywords

  • Amplification refractory mutation system-PCR
  • Clarithromycin
  • erm(41)
  • Real-time PCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)

Cite this

Detection and assessment of clarithromycin inducible resistant strains among Korean mycobacterium abscessus clinical strains : PCR methods. / Lee, Seung Heon; Yoo, Hee Kyung; Kim, Seol Hee; Koh, Won Jung; Kim, Chang Ki; Park, Young Kil; Kim, Hee Jin.

In: Journal of Clinical Laboratory Analysis, Vol. 28, No. 5, 01.01.2014, p. 409-414.

Research output: Contribution to journalArticle

Lee, Seung Heon ; Yoo, Hee Kyung ; Kim, Seol Hee ; Koh, Won Jung ; Kim, Chang Ki ; Park, Young Kil ; Kim, Hee Jin. / Detection and assessment of clarithromycin inducible resistant strains among Korean mycobacterium abscessus clinical strains : PCR methods. In: Journal of Clinical Laboratory Analysis. 2014 ; Vol. 28, No. 5. pp. 409-414.
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abstract = "Background: Mycobacterium abscessus group belongs to a group of rapidly growing mycobacteria (RGM) and, following Mycobacterium avium complex, is the second most common pathogen responsible for lung disease caused by nontuberculous mycobacteria (NTM). Clarithromycin is known to be the key drug in the treatment of M. abscessus group disease, but a high failure rate of treatment response is reported due to clarithromycin inducible resistance. Methods: Using the results from a clarithromycin susceptibility test we examined the proportion of clarithromycin inducible resistant M. abscessus (sensu stricto; hereafter referred to as M. abscessus) clinical strains. Also, we attempted to detect the clarithromycin resistant strains, using the amplification refractory mutation system-PCR (ARMS-PCR) and real-time PCR methods for rapid detection of single-nucleotide polymorphisms (SNPs) at position 28 (T or C) of the erm(41) gene of M. abscessus leading to resistance to clarithromycin. Results: Of the 157 M. abscessus clinical strains, clarithromycin susceptible, resistant, and inducible resistant strains accounted for 10.83{\%} (n = 17), 22.29{\%} (n = 35), and 66.88{\%} (n = 105), respectively. Clarithromycin resistant strains were able to separate from clarithromycin susceptible strains by ARMS-PCR and real-time PCR identical to DNA sequence analysis. Conclusion: Most M. abscessus clinical strains in Korea are resistant to clarithromycin, and ARMS-PCR and real-time PCR are useful tools for the rapid detection of single-nucleotide polymorphisms (SNPs) at position 28 of the erm(41) gene.",
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T1 - Detection and assessment of clarithromycin inducible resistant strains among Korean mycobacterium abscessus clinical strains

T2 - PCR methods

AU - Lee, Seung Heon

AU - Yoo, Hee Kyung

AU - Kim, Seol Hee

AU - Koh, Won Jung

AU - Kim, Chang Ki

AU - Park, Young Kil

AU - Kim, Hee Jin

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: Mycobacterium abscessus group belongs to a group of rapidly growing mycobacteria (RGM) and, following Mycobacterium avium complex, is the second most common pathogen responsible for lung disease caused by nontuberculous mycobacteria (NTM). Clarithromycin is known to be the key drug in the treatment of M. abscessus group disease, but a high failure rate of treatment response is reported due to clarithromycin inducible resistance. Methods: Using the results from a clarithromycin susceptibility test we examined the proportion of clarithromycin inducible resistant M. abscessus (sensu stricto; hereafter referred to as M. abscessus) clinical strains. Also, we attempted to detect the clarithromycin resistant strains, using the amplification refractory mutation system-PCR (ARMS-PCR) and real-time PCR methods for rapid detection of single-nucleotide polymorphisms (SNPs) at position 28 (T or C) of the erm(41) gene of M. abscessus leading to resistance to clarithromycin. Results: Of the 157 M. abscessus clinical strains, clarithromycin susceptible, resistant, and inducible resistant strains accounted for 10.83% (n = 17), 22.29% (n = 35), and 66.88% (n = 105), respectively. Clarithromycin resistant strains were able to separate from clarithromycin susceptible strains by ARMS-PCR and real-time PCR identical to DNA sequence analysis. Conclusion: Most M. abscessus clinical strains in Korea are resistant to clarithromycin, and ARMS-PCR and real-time PCR are useful tools for the rapid detection of single-nucleotide polymorphisms (SNPs) at position 28 of the erm(41) gene.

AB - Background: Mycobacterium abscessus group belongs to a group of rapidly growing mycobacteria (RGM) and, following Mycobacterium avium complex, is the second most common pathogen responsible for lung disease caused by nontuberculous mycobacteria (NTM). Clarithromycin is known to be the key drug in the treatment of M. abscessus group disease, but a high failure rate of treatment response is reported due to clarithromycin inducible resistance. Methods: Using the results from a clarithromycin susceptibility test we examined the proportion of clarithromycin inducible resistant M. abscessus (sensu stricto; hereafter referred to as M. abscessus) clinical strains. Also, we attempted to detect the clarithromycin resistant strains, using the amplification refractory mutation system-PCR (ARMS-PCR) and real-time PCR methods for rapid detection of single-nucleotide polymorphisms (SNPs) at position 28 (T or C) of the erm(41) gene of M. abscessus leading to resistance to clarithromycin. Results: Of the 157 M. abscessus clinical strains, clarithromycin susceptible, resistant, and inducible resistant strains accounted for 10.83% (n = 17), 22.29% (n = 35), and 66.88% (n = 105), respectively. Clarithromycin resistant strains were able to separate from clarithromycin susceptible strains by ARMS-PCR and real-time PCR identical to DNA sequence analysis. Conclusion: Most M. abscessus clinical strains in Korea are resistant to clarithromycin, and ARMS-PCR and real-time PCR are useful tools for the rapid detection of single-nucleotide polymorphisms (SNPs) at position 28 of the erm(41) gene.

KW - Amplification refractory mutation system-PCR

KW - Clarithromycin

KW - erm(41)

KW - Real-time PCR

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