Detection and typing of respiratory adenoviruses in a single-tube multiplex polymerase chain reaction

Byoung Kuk Na, Jee Hee Kim, Gu Choul Shin, Joo Yeon Lee, Jin Soo Lee, Chun Kang, Woo Joo Kim

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A multiplex polymerase chain reaction (PCR) assay that is capable of detecting and typing six serotypes of respiratory adenovirus (Ad) was developed, using multiple sets of type-specific primers. The detection of each different serotype depended on distinguishing different numbers and sizes of amplification products on agarose gels following PCR. The multiplex PCR was tested with 26 clinical Ad isolates and other respiratory viruses including influenza viruses, parainfluenza viruses, and respiratory syncytial viruses as well as respiratory bacterial pathogens such as Chlamydia pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, and Mycoplasma pneumoniae. The multiplex PCR for the detection and typing of Ads gave an excellent correlation with the results by conventional typing with type-specific antisera. This assay may serve as a rapid means of confirming Ad with simultaneous serotype identification of the isolates. It will also have relevance as an adjunctive tool to conventional serotyping for diagnostic and epidemiological purposes.

Original languageEnglish
Pages (from-to)512-517
Number of pages6
JournalJournal of Medical Virology
Volume66
Issue number4
DOIs
Publication statusPublished - 2002

Keywords

  • Adenoviruses
  • Detection
  • Multiplex PCR
  • Typing

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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