Detection, antibiotic susceptibility and biofilm formation of Cronobacter spp. from various foods in Korea

Young Duck Lee, Jong Hyun Park, Hyo Ihl Chang

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Cronobacter spp. are emerging food-borne pathogens and can lead to serious developmental aftereffects and death in infants. Foods contaminated with Cronobacter spp. may pose a high risk to infants or weak individuals. In Korea, 353 various types of foods were examined for the prevalence of Cronobacter spp. Isolates were confirmed by targeting the ompA gene and the tRNA-23s rRNA intergenic region using VITEK and PCR. The results of a 16S rDNA gene sequence analysis showed 58 isolates of Cronobacter sakazakii, 2 isolates of Cronobacter muytjensii, and 6 isolates of Cronobacter dublinensis. The isolates were found to be susceptible to 4 antibiotics on performing agar disk diffusion. C. sakazakii isolates were susceptible to ampicillin(98.2%), tetracycline(98.2%), and chloramphenicol(96.6%) but resistant to streptomycin(12.0%). C. muytjensii and C. dublinensis isolates were susceptible to ampicillin, tetracycline and chloramphenicol. Biofilm formation by microtiter plate assay varied greatly between different Cronobacter spp. isolates. Cronobacter spp. isolates were frequently detected in various foods, and the most prevalent strain was C. sakazakii. Cronobacter spp. isolates did not seem to differ in terms of antibiotic susceptibility and biofilm formation.

Original languageEnglish
Pages (from-to)225-230
Number of pages6
JournalFood Control
Volume24
Issue number1-2
DOIs
Publication statusPublished - 2012 Mar

Keywords

  • Antibiotics susceptibility
  • Biofilm formation
  • Cronobacter spp.
  • Detection

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

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