Detection of bisphenol A using palm-size NanoAptamer analyzer

Hyun Jeong Lim, Beelee Chua, Ahjeong Son

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have demonstrated a palm-size NanoAptamer analyzer capable of detecting bisphenol A (BPA) at environmentally relevant concentrations (<1 ng/mL or ppb). It is designed for performing reaction and fluorescence measurement on single cuvette sample. Modified NanoGene assay was used as the sensing mechanism where signaling DNA and QD655 was tethered to QD565 and magnetic bead via the aptamer. Aptamer affinity with BPA resulted in the release of the signaling DNA and QD655 from the complex and hence corresponding decrease in QD655 fluorescence measurement signal. Baseline characterization was first performed with empty cuvettes, quantum dots and magnetic beads under near-ideal conditions to establish essential functionality of the NanoAptamer analyzer. Duration of incubation time, number of rinse cycles, and necessity of cuvette vibration were also investigated. In order to demonstrate the capability of the NanoAptamer analyzer to detect BPA, samples with BPA concentrations ranging from 0.0005 to 1.0 ng/mL (ppb) were used. The performance of the NanoAptamer analyzer was further examined by using laboratory protocol and commercial spectrofluorometer as reference. Correlation between NanoAptamer analyzer and laboratory protocol as well as commercial spectrofluorometer was evaluated via correlation plots and correlation coefficients.

Original languageEnglish
Pages (from-to)10-18
Number of pages9
JournalBiosensors and Bioelectronics
Volume94
DOIs
Publication statusPublished - 2017 Aug 15

Fingerprint

DNA
Fluorescence
Semiconductor quantum dots
Assays
Quantum Dots
Vibration
bisphenol A

Keywords

  • Aptamer
  • Bisphenol A (BPA)
  • Fluorescence measurement
  • Magnetic bead
  • NanoAptamer analyzer
  • Quantum dots

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Detection of bisphenol A using palm-size NanoAptamer analyzer. / Lim, Hyun Jeong; Chua, Beelee; Son, Ahjeong.

In: Biosensors and Bioelectronics, Vol. 94, 15.08.2017, p. 10-18.

Research output: Contribution to journalArticle

Lim, Hyun Jeong ; Chua, Beelee ; Son, Ahjeong. / Detection of bisphenol A using palm-size NanoAptamer analyzer. In: Biosensors and Bioelectronics. 2017 ; Vol. 94. pp. 10-18.
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