Detection of clarithromycin-resistant strains from clinical isolates of Mycobacterium abscessus

Seung Heon Lee, Young Kil Park, Sung Weon Ryo, Myung Sup Shim, Woo Jin Lew, Hee Jin Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Mycobacterium abscessus is the most pathogenic and drug-resistant rapid-growing mycobacterium. Clarithromycin or azithromycin are the only regular oral antimycobacterial agents that have an effect on M. abscessus. We tried to detect the clarithromycin-resistant strains from the clinical isolates of M. abscessus. Methods: We tried to isolate the clarithromycin-resistant strains from 220 clinical isolates of M. abscessus by performing using reverse hybridization assay (RHA) and the broth microdilution test (BMT). Results: Seven resistant strains (3.2%) from all the tested clinical isolates were detected by BMT. Three of these resistant strains were also detected by RHA and it was confirmed that they had point mutants. Conclusion: These results showed that clarithromycin resistance in M. abscessus clinical isolates is related to a point mutation and other unknown mechanisms.

Original languageEnglish
Pages (from-to)422-426
Number of pages5
JournalTuberculosis and Respiratory Diseases
Volume64
Issue number6
DOIs
Publication statusPublished - 2008 Jun 1
Externally publishedYes

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Clarithromycin
Mycobacterium
Azithromycin
Point Mutation
Anti-Bacterial Agents
Pharmaceutical Preparations

Keywords

  • 23S rRNA
  • Broth microdilution test
  • Clarithromycin
  • Mycobacterium abscessus
  • Reverse hybridization assay

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Cite this

Detection of clarithromycin-resistant strains from clinical isolates of Mycobacterium abscessus. / Lee, Seung Heon; Park, Young Kil; Ryo, Sung Weon; Shim, Myung Sup; Lew, Woo Jin; Kim, Hee Jin.

In: Tuberculosis and Respiratory Diseases, Vol. 64, No. 6, 01.06.2008, p. 422-426.

Research output: Contribution to journalArticle

Lee, Seung Heon ; Park, Young Kil ; Ryo, Sung Weon ; Shim, Myung Sup ; Lew, Woo Jin ; Kim, Hee Jin. / Detection of clarithromycin-resistant strains from clinical isolates of Mycobacterium abscessus. In: Tuberculosis and Respiratory Diseases. 2008 ; Vol. 64, No. 6. pp. 422-426.
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