Detection of clarithromycin-resistant strains from clinical isolates of Mycobacterium abscessus

Seung Heon Lee, Young Kil Park, Sung Weon Ryo, Myung Sup Shim, Woo Jin Lew, Hee Jin Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background: Mycobacterium abscessus is the most pathogenic and drug-resistant rapid-growing mycobacterium. Clarithromycin or azithromycin are the only regular oral antimycobacterial agents that have an effect on M. abscessus. We tried to detect the clarithromycin-resistant strains from the clinical isolates of M. abscessus. Methods: We tried to isolate the clarithromycin-resistant strains from 220 clinical isolates of M. abscessus by performing using reverse hybridization assay (RHA) and the broth microdilution test (BMT). Results: Seven resistant strains (3.2%) from all the tested clinical isolates were detected by BMT. Three of these resistant strains were also detected by RHA and it was confirmed that they had point mutants. Conclusion: These results showed that clarithromycin resistance in M. abscessus clinical isolates is related to a point mutation and other unknown mechanisms.

Original languageEnglish
Pages (from-to)422-426
Number of pages5
JournalTuberculosis and Respiratory Diseases
Volume64
Issue number6
DOIs
Publication statusPublished - 2008 Jun
Externally publishedYes

Keywords

  • 23S rRNA
  • Broth microdilution test
  • Clarithromycin
  • Mycobacterium abscessus
  • Reverse hybridization assay

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

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