Detection of genetic alterations in Korean ovarian carcinomas by degenerate oligonucleotide primed polymerase chain reaction-comparative genomic hybridization

Gi Jin Kim, Joo Oh Kim, Eun Kyung Hong, Hyun Kim, Yong Hyuck Chun, Sun-Hwa Park

Research output: Contribution to journalArticle

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Abstract

Chromosomal aberrations in 22 Koreans with ovarian carcinomas were investigated by degenerate oligonucleotide primed-polymerase chain reaction comparative genomic hybridization. The common sites of copy number increases were 20q (90%), 17q23∼qter (86%), 8q22∼qter (68%), 3q25∼qter (59%), 6p21 (59%), 11q13 (54%), 16p (40%), 2q31∼qter (36%), 7q (36%), 14q31 (36%), 15q24∼qter (36%), and 1q32∼qter (31%). DNA amplification was identified in 18 carcinomas (82%). The frequent sites of amplification were 20q13.2∼qter, 8q24.1, 17q23∼qter, 3q25∼qter, and 6p21. The most frequent sites of copy number decreases were 4q21∼q31 (54%), 5q13∼q21 (50%), and 13q14∼q21 (45%). The recurrent gains and losses of chromosomal regions identified in this study provide candidate regions that may contain oncogenes or tumor suppressor genes, respectively.

Original languageEnglish
Pages (from-to)23-27
Number of pages5
JournalCancer Genetics and Cytogenetics
Volume147
Issue number1
DOIs
Publication statusPublished - 2003 Nov 1

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Comparative Genomic Hybridization
Carcinoma
Polymerase Chain Reaction
Tumor Suppressor Genes
Oncogenes
Chromosome Aberrations
DNA
ribonucleotide polymerase

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Detection of genetic alterations in Korean ovarian carcinomas by degenerate oligonucleotide primed polymerase chain reaction-comparative genomic hybridization. / Kim, Gi Jin; Kim, Joo Oh; Hong, Eun Kyung; Kim, Hyun; Chun, Yong Hyuck; Park, Sun-Hwa.

In: Cancer Genetics and Cytogenetics, Vol. 147, No. 1, 01.11.2003, p. 23-27.

Research output: Contribution to journalArticle

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abstract = "Chromosomal aberrations in 22 Koreans with ovarian carcinomas were investigated by degenerate oligonucleotide primed-polymerase chain reaction comparative genomic hybridization. The common sites of copy number increases were 20q (90{\%}), 17q23∼qter (86{\%}), 8q22∼qter (68{\%}), 3q25∼qter (59{\%}), 6p21 (59{\%}), 11q13 (54{\%}), 16p (40{\%}), 2q31∼qter (36{\%}), 7q (36{\%}), 14q31 (36{\%}), 15q24∼qter (36{\%}), and 1q32∼qter (31{\%}). DNA amplification was identified in 18 carcinomas (82{\%}). The frequent sites of amplification were 20q13.2∼qter, 8q24.1, 17q23∼qter, 3q25∼qter, and 6p21. The most frequent sites of copy number decreases were 4q21∼q31 (54{\%}), 5q13∼q21 (50{\%}), and 13q14∼q21 (45{\%}). The recurrent gains and losses of chromosomal regions identified in this study provide candidate regions that may contain oncogenes or tumor suppressor genes, respectively.",
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