Detection of glyphosate by quantitative analysis of fluorescence and single DNA using DNA-labeled fluorescent magnetic core-shell nanoparticles

Hee Uk Lee, Da Un Jung, Ja Hyun Lee, Yoon Seok Song, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Biosensors based on antibody recognition have a wide range of monitoring applications with applications to environmental and food problems. In an effort to improve the limit of detection, novel Co-B/SiO2/dye nanoparticles were prepared in a water-in-oil microemulsion and subsequently surface modified. The fluorescence magnetic nanoparticle (FMP) based detection method for quantitatively fluorescence intensity and specific DNA-based application is described. A sandwich format consisting of conjugated glyphosate-double target/probe DNA-FMP was fabricated. Results were measured based on the fluorescence intensity obtained by comparing the free glyphosates at concentrations of 1-10,000 nM for detecting the binding of glyphosate-double DNA-FMP nanoparticles. Moreover, quantitative information concerning the free glyphosate analysis was translated into a single probe DNA signal with a good linear correlation in the concentration range of 1-10,000 nM (R2 = 0.98) and a limit of detection of 0.27 nM. Therefore, detection of glyphosate could easily and quantitatively be measured using confocal laser scanning microscopy and low-cost UV photometric analysis. These results indicate that this method is a very simple and economic strategy for detecting glyphosate.

Original languageEnglish
Pages (from-to)879-886
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume177
DOIs
Publication statusPublished - 2013

Keywords

  • Fluorescence
  • Glyphosate
  • Immunosensor
  • Magnetic nanoparticle
  • Quantitative analysis
  • Water-in-oil

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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