Abstract
Background and Aims: The Detection of hepatitis A virus (HAV) is important for diagnosis and epidemiological studies of hepatitis A. The polymerase chain reaction (PCR) technique is a sensitive test to detect HAV-RNA in specimens. The aims of the present study were to clarify the detection rate of serum HAV-RNA by PCR and the natural history of HAV viraemia, and to determine the correlation between viraemia and the clinical characteristics in patients with acute hepatitis A. Methods: Hepatitis A virus RNA was tested in 74 serum samples which were serially collected from 27 patients with acute hepatitis A. A nested reverse transcription (RT)-PCR for HAV-RNA was performed with primer sets located at the VP1 region of the HAV genome and the PCR products were eletrophoresed on a 1.5% agarose gel. Results: Hepatitis A virus RNA was found in 18 of 27 (67%) patients with hepatitis A. There were no significant differences between groups positive and negative for HAV-RNA in clinical and laboratory data, except the time interval between clinical onset and initial serum sampling for RT-PCR (10 ± 6 vs 19 ± 14 days) and the alanine aminotransferase (ALT) level at initial serum sampling for RT-PCR (1436 ±1416 vs 518 ± 432 IU/L). The mean duration of HAV viraemia was 30 ±19 days (range, 5-59 days). The duration of HAV viraemia and duration of abnormal ALT levels from clinical onset were positively correlated (r= 0.685, P= 0.007). Conclusion: In conclusion, HAV-RNA RT-PCR is a useful tool to detect HAV viraemia and to study the molecular epidemiology of HAV infection. (C) 2000 Blackwell Science Asia Pty Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 1043-1047 |
| Number of pages | 5 |
| Journal | Journal of Gastroenterology and Hepatology (Australia) |
| Volume | 15 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2000 Jan 1 |
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Keywords
- Hepatitis A
- Hepatitis A virus RNA
- Serum
- Viraemia
ASJC Scopus subject areas
- Hepatology
- Gastroenterology
Cite this
Detection of hepatitis A viral RNA in sera of patients with acute hepatitis A. / Kwon, Oh Sang; Byun, Kwan Soo; Yeon, Jong Eun; Park, Sang Hoon; Kim, Jae Seon; Kim, Ju Hyun; Bak, Young-Tae; Kim, Jin Ho; Lee, Chang Hong.
In: Journal of Gastroenterology and Hepatology (Australia), Vol. 15, No. 9, 01.01.2000, p. 1043-1047.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of hepatitis A viral RNA in sera of patients with acute hepatitis A
AU - Kwon, Oh Sang
AU - Byun, Kwan Soo
AU - Yeon, Jong Eun
AU - Park, Sang Hoon
AU - Kim, Jae Seon
AU - Kim, Ju Hyun
AU - Bak, Young-Tae
AU - Kim, Jin Ho
AU - Lee, Chang Hong
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Background and Aims: The Detection of hepatitis A virus (HAV) is important for diagnosis and epidemiological studies of hepatitis A. The polymerase chain reaction (PCR) technique is a sensitive test to detect HAV-RNA in specimens. The aims of the present study were to clarify the detection rate of serum HAV-RNA by PCR and the natural history of HAV viraemia, and to determine the correlation between viraemia and the clinical characteristics in patients with acute hepatitis A. Methods: Hepatitis A virus RNA was tested in 74 serum samples which were serially collected from 27 patients with acute hepatitis A. A nested reverse transcription (RT)-PCR for HAV-RNA was performed with primer sets located at the VP1 region of the HAV genome and the PCR products were eletrophoresed on a 1.5% agarose gel. Results: Hepatitis A virus RNA was found in 18 of 27 (67%) patients with hepatitis A. There were no significant differences between groups positive and negative for HAV-RNA in clinical and laboratory data, except the time interval between clinical onset and initial serum sampling for RT-PCR (10 ± 6 vs 19 ± 14 days) and the alanine aminotransferase (ALT) level at initial serum sampling for RT-PCR (1436 ±1416 vs 518 ± 432 IU/L). The mean duration of HAV viraemia was 30 ±19 days (range, 5-59 days). The duration of HAV viraemia and duration of abnormal ALT levels from clinical onset were positively correlated (r= 0.685, P= 0.007). Conclusion: In conclusion, HAV-RNA RT-PCR is a useful tool to detect HAV viraemia and to study the molecular epidemiology of HAV infection. (C) 2000 Blackwell Science Asia Pty Ltd.
AB - Background and Aims: The Detection of hepatitis A virus (HAV) is important for diagnosis and epidemiological studies of hepatitis A. The polymerase chain reaction (PCR) technique is a sensitive test to detect HAV-RNA in specimens. The aims of the present study were to clarify the detection rate of serum HAV-RNA by PCR and the natural history of HAV viraemia, and to determine the correlation between viraemia and the clinical characteristics in patients with acute hepatitis A. Methods: Hepatitis A virus RNA was tested in 74 serum samples which were serially collected from 27 patients with acute hepatitis A. A nested reverse transcription (RT)-PCR for HAV-RNA was performed with primer sets located at the VP1 region of the HAV genome and the PCR products were eletrophoresed on a 1.5% agarose gel. Results: Hepatitis A virus RNA was found in 18 of 27 (67%) patients with hepatitis A. There were no significant differences between groups positive and negative for HAV-RNA in clinical and laboratory data, except the time interval between clinical onset and initial serum sampling for RT-PCR (10 ± 6 vs 19 ± 14 days) and the alanine aminotransferase (ALT) level at initial serum sampling for RT-PCR (1436 ±1416 vs 518 ± 432 IU/L). The mean duration of HAV viraemia was 30 ±19 days (range, 5-59 days). The duration of HAV viraemia and duration of abnormal ALT levels from clinical onset were positively correlated (r= 0.685, P= 0.007). Conclusion: In conclusion, HAV-RNA RT-PCR is a useful tool to detect HAV viraemia and to study the molecular epidemiology of HAV infection. (C) 2000 Blackwell Science Asia Pty Ltd.
KW - Hepatitis A
KW - Hepatitis A virus RNA
KW - Serum
KW - Viraemia
UR - http://www.scopus.com/inward/record.url?scp=0033784139&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033784139&partnerID=8YFLogxK
U2 - 10.1046/j.1440-1746.2000.02291.x
DO - 10.1046/j.1440-1746.2000.02291.x
M3 - Article
C2 - 11059935
AN - SCOPUS:0033784139
VL - 15
SP - 1043
EP - 1047
JO - Journal of Gastroenterology and Hepatology (Australia)
JF - Journal of Gastroenterology and Hepatology (Australia)
SN - 0815-9319
IS - 9
ER -